Chinese Journal of Oceanology and Limnology

, Volume 30, Issue 5, pp 786–790

A new simple screening method for the detection of paralytic shellfish poisoning toxins

  • Jinping Cheng (程金平)
  • Shuaishuai Pi (皮帅帅)
  • Shufeng Ye (叶属峰)
  • Haomin Gao (高昊旻)
  • Lei Yao (姚磊)
  • Zhenyi Jiang (蒋真毅)
  • Yuling Song (宋玉玲)
  • Lei Xi (席磊)
Biology

DOI: 10.1007/s00343-012-1097-8

Cite this article as:
Cheng, J., Pi, S., Ye, S. et al. Chin. J. Ocean. Limnol. (2012) 30: 786. doi:10.1007/s00343-012-1097-8

Abstract

The current testing for paralytic shellfish poisoning (PSP) in shellfish is based on the mouse bioassay (MBA). To alleviate animal welfare concerns, we evaluated the utility of using sublethal indicators of toxicity as an alternative to measuring time to death. Live mice were injected with a PSP congener and the changes in neurotransmitter levels were measured 60, 90, and 120 min after injection. Acetylcholine (ACh) was the most sensitive marker for PSP toxicity. The changes in neurotransmitter levels were most pronounced in the blood. Thus, measurement of Ach levels in the blood may serve as a sensitive predictor for PSP that would not require sacrifice of the mice. This method was relatively simple, sensitive (less than 1 μg/kg weight, equivalent to 20 ng/mL), low maintenance, and rapid (less than 60 min).

Keyword

paralytic shellfish poisoning (PSP) mouse bioassay acetylcholine assay 

Copyright information

© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • Jinping Cheng (程金平)
    • 1
  • Shuaishuai Pi (皮帅帅)
    • 1
  • Shufeng Ye (叶属峰)
    • 2
  • Haomin Gao (高昊旻)
    • 3
  • Lei Yao (姚磊)
    • 3
  • Zhenyi Jiang (蒋真毅)
    • 3
  • Yuling Song (宋玉玲)
    • 1
  • Lei Xi (席磊)
    • 1
  1. 1.School of Environmental Science and EngineeringShanghai Jiao Tong UniversityShanghaiChina
  2. 2.Key Laboratory of Marine Integrated Monitoring and Applied Technologies of Harmful Algal BloomsChinese State Oceanic AdministrationShanghaiChina
  3. 3.Shanghai Municipal Oceanic BureauShanghaiChina

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