Chinese Journal of Oceanology and Limnology

, Volume 28, Issue 1, pp 26–36

Analysis of expressed sequence tags from the Ulva prolifera (Chlorophyta)

  • Jianfeng Niu牛建峰
  • Haiyan Hu胡海岩
  • Songnian Hu胡松年
  • Guangce Wang王广策
  • Guang Peng彭光
  • Song Sun孙 松
Biology

DOI: 10.1007/s00343-010-9120-4

Cite this article as:
Niu, J., Hu, H., Hu, S. et al. Chin. J. Ocean. Limnol. (2010) 28: 26. doi:10.1007/s00343-010-9120-4

Abstract

In 2008, a green tide broke out before the sailing competition of the 29th Olympic Games in Qingdao. The causative species was determined to be Enteromorpha prolifera (Ulva prolifera O. F. Müller), a familiar green macroalga along the coastline of China. Rapid accumulation of a large biomass of floating U. prolifera prompted research on different aspects of this species. In this study, we constructed a nonnormalized cDNA library from the thalli of U. prolifera and acquired 10 072 high-quality expressed sequence tags (ESTs). These ESTs were assembled into 3 519 nonredundant gene groups, including 1 446 clusters and 2 073 singletons. After annotation with the nr database, a large number of genes were found to be related with chloroplast and ribosomal protein, GO functional classification showed 1 418 ESTs participated in photosynthesis and 1 359 ESTs were responsible for the generation of precursor metabolites and energy. In addition, rather comprehensive carbon fixation pathways were found in U. prolifera using KEGG. Some stress-related and signal transduction-related genes were also found in this study. All the evidences displayed that U. prolifera had substance and energy foundation for the intense photosynthesis and the rapid proliferation. Phylogenetic analysis of cytochrome c oxidase subunit I revealed that this green-tide causative species is most closely affiliated to Pseudendoclonium akinetum (Ulvophyceae).

Keyword

green tideUlva Proliferaexpressed sequence tag (EST)filamentous green algarapid growthproliferation

Copyright information

© Chinese Society for Oceanology and Limnology, Science Press and Springer Berlin Heidelberg 2010

Authors and Affiliations

  • Jianfeng Niu牛建峰
    • 1
  • Haiyan Hu胡海岩
    • 2
    • 4
  • Songnian Hu胡松年
    • 2
  • Guangce Wang王广策
    • 1
    • 3
  • Guang Peng彭光
    • 1
  • Song Sun孙 松
    • 1
  1. 1.Key Laboratory of Experimental Marine Biology, Institute of OceanologyChinese Academy of SciencesQingdaoChina
  2. 2.Key Laboratory of Genome Sciences and Information, Beijing Institute of GenomicsChinese Academy of SciencesBeijingChina
  3. 3.College of Marine Science and EngineeringTianjin University of Science and TechnologyTianjinChina
  4. 4.Graduate University of Chinese Academy of SciencesBeijingChina