Chemistry

Chinese Journal of Oceanology and Limnology

, Volume 27, Issue 3, pp 578-582

First online:

Analysis of the monosaccharide composition of fucoidan by precolumn derivation HPLC

  • Jingjing Zhang张晶晶Affiliated withInstitute of Oceanology, Chinese Academy of SciencesGraduate School of Chinese Academy of Sciences
  • , Quanbin Zhang张全斌Affiliated withInstitute of Oceanology, Chinese Academy of Sciences Email author 
  • , Jing Wang王晶Affiliated withInstitute of Oceanology, Chinese Academy of SciencesGraduate School of Chinese Academy of Sciences
  • , Xuelian Shi石学连Affiliated withInstitute of Oceanology, Chinese Academy of SciencesGraduate School of Chinese Academy of Sciences
  • , Zhongshan Zhang张忠山Affiliated withInstitute of Oceanology, Chinese Academy of SciencesGraduate School of Chinese Academy of Sciences

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Abstract

We developed an HPLC method for analysis of the monosaccharide composition of fucoidans. The fucoidan was hydrolyzed into monosaccharides with 2 mol/L trifluoroacetic acid. Using ribose as the internal standard, the monosaccharide derivatives, obtained with 1-Phenyl-3-methyl-5-pyrazolone (PMP), were separated by reverse-phase HPLC using a gradient elution process, and monitored by ultraviolet detection at 245 nm. In the concentration range of 0.1–2.0 mmol/L, the peak area of each monosaccharide had a good linear relationship with its concentration (r 2>0.998). The average recoveries of mannose, rhamnose, glucuronic acid, glucose, galactose, xylose, and fucose were 86.2%, 95.1%, 62.5%, 102.0%, 94.8%, 66.6%, and 105.1%, respectively. This method was accurate and had good reproducibility and could be used to determine the monosaccharide contents of fucoidans.

Keyword

fucoidan monosaccharide derivation HPLC