Chinese Journal of Oceanology and Limnology

, Volume 27, Issue 3, pp 578–582

Analysis of the monosaccharide composition of fucoidan by precolumn derivation HPLC

Authors

  • Jingjing Zhang张晶晶
    • Institute of OceanologyChinese Academy of Sciences
    • Graduate School of Chinese Academy of Sciences
    • Institute of OceanologyChinese Academy of Sciences
  • Jing Wang王晶
    • Institute of OceanologyChinese Academy of Sciences
    • Graduate School of Chinese Academy of Sciences
  • Xuelian Shi石学连
    • Institute of OceanologyChinese Academy of Sciences
    • Graduate School of Chinese Academy of Sciences
  • Zhongshan Zhang张忠山
    • Institute of OceanologyChinese Academy of Sciences
    • Graduate School of Chinese Academy of Sciences
Chemistry

DOI: 10.1007/s00343-009-9205-0

Cite this article as:
Zhang, J., Zhang, Q., Wang, J. et al. Chin. J. Ocean. Limnol. (2009) 27: 578. doi:10.1007/s00343-009-9205-0

Abstract

We developed an HPLC method for analysis of the monosaccharide composition of fucoidans. The fucoidan was hydrolyzed into monosaccharides with 2 mol/L trifluoroacetic acid. Using ribose as the internal standard, the monosaccharide derivatives, obtained with 1-Phenyl-3-methyl-5-pyrazolone (PMP), were separated by reverse-phase HPLC using a gradient elution process, and monitored by ultraviolet detection at 245 nm. In the concentration range of 0.1–2.0 mmol/L, the peak area of each monosaccharide had a good linear relationship with its concentration (r2>0.998). The average recoveries of mannose, rhamnose, glucuronic acid, glucose, galactose, xylose, and fucose were 86.2%, 95.1%, 62.5%, 102.0%, 94.8%, 66.6%, and 105.1%, respectively. This method was accurate and had good reproducibility and could be used to determine the monosaccharide contents of fucoidans.

Keyword

fucoidanmonosaccharidederivationHPLC

Copyright information

© Chinese Society for Oceanology and Limnology, Science Press and Springer Berlin Heidelberg 2009