Applied Physics B

, Volume 73, Issue 8, pp 869–871

Evanescent-field-induced two-photon fluorescence: excitation of macroscopic areas of planar waveguides

  • G.L. Duveneck
  • M.A. Bopp
  • M. Ehrat
  • M. Haiml
  • U. Keller
  • M.A. Bader
  • G. Marowsky
  • S. Soria
Rapid communication

DOI: 10.1007/s003400100773

Cite this article as:
Duveneck, G., Bopp, M., Ehrat, M. et al. Appl Phys B (2001) 73: 869. doi:10.1007/s003400100773

Abstract.

Two-photon excitation (TPE) of fluorescence is a powerful tool for separating a faintly emitted fluorescence signal from background excitation noise. Until now TPE has only been accomplished for very small excitation areas of a few square micrometre dimensions since they are readily available in the focal zone of high-power lasers. In this paper we demonstrate, to our knowledge for the first time, two-photon excited fluorescence with planar thin-film waveguide structures of macroscopic excitation areas of the order of square millimetres to square centimetres.

Based upon our results, it can be envisaged that the new combination of planar waveguide technology with TPE will become a powerful tool for macroscopic surface-interaction studies. It can also open the way for further improving the sensitivity of biosensing platforms like genomic and proteomic microarrays based upon planar waveguides.

PACS: 87.50.Hj; 42.62.Be; 42.79.Gn

Copyright information

© Springer-Verlag 2001

Authors and Affiliations

  • G.L. Duveneck
    • 1
  • M.A. Bopp
    • 1
  • M. Ehrat
    • 1
  • M. Haiml
    • 2
  • U. Keller
    • 2
  • M.A. Bader
    • 3
  • G. Marowsky
    • 3
  • S. Soria
    • 3
  1. 1.Zeptosens AG, Benkenstrasse 254, 4108 Witterswil, SwitzerlandSwitzerland
  2. 2.Institute of Quantum Electronics, Swiss Federal Institute of Technology, ETH Hönggerberg, HPT, 8093 Zürich, SwitzerlandSwitzerland
  3. 3.Laser-Laboratorium Göttingen e.V., Hans-Adolf-Krebs Weg 1, 37077 Göttingen, GermanyGermany