Applied Physics B

, Volume 74, Issue 1, pp 95–99

Detection of biogenic CO production above vascular cell cultures using a near-room- temperature QC-DFB laser

  • A.A. Kosterev
  • F.K. Tittel
  • W. Durante
  • M. Allen
  • R. Köhler
  • C. Gmachl
  • F. Capasso
  • D.L. Sivco
  • A.Y. Cho

DOI: 10.1007/s003400100766

Cite this article as:
Kosterev, A., Tittel, F., Durante, W. et al. Appl Phys B (2002) 74: 95. doi:10.1007/s003400100766

Abstract.

We report the first application of pulsed, near-room-temperature quantum cascade laser technology to the continuous detection of biogenic CO production rates above viable cultures of vascular smooth muscle cells. A computer-controlled sequence of measurements over a 9-h period was obtained, resulting in a minimum detectable CO production of 20 ppb in a 1-m optical path above a standard cell-culture flask. Data-processing procedures for real-time monitoring of both biogenic and ambient atmospheric CO concentrations are described.

PACS: 42.62.Be; 82.80.Gk

Copyright information

© Springer-Verlag 2001

Authors and Affiliations

  • A.A. Kosterev
    • 1
  • F.K. Tittel
    • 1
  • W. Durante
    • 2
  • M. Allen
    • 3
  • R. Köhler
    • 4
  • C. Gmachl
    • 4
  • F. Capasso
    • 4
  • D.L. Sivco
    • 4
  • A.Y. Cho
    • 4
  1. 1.Rice Quantum Institute, Rice University, Houston, TX 77 251-1892, USAUS
  2. 2.Departments of Medicine and Pharmacology, Baylor College of Medicine, Houston, TX 77 030, USAUS
  3. 3.Physical Sciences Inc., 20 New England Business Center, Andover, MA 01810, USAUS
  4. 4.Bell Laboratories, Lucent Technologies, 600 Mountain Avenue, Murray Hill, NJ 07974, USAUS