Detection of biogenic CO production above vascular cell cultures using a near-room- temperature QC-DFB laser
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- Kosterev, A., Tittel, F., Durante, W. et al. Appl Phys B (2002) 74: 95. doi:10.1007/s003400100766
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We report the first application of pulsed, near-room-temperature quantum cascade laser technology to the continuous detection of biogenic CO production rates above viable cultures of vascular smooth muscle cells. A computer-controlled sequence of measurements over a 9-h period was obtained, resulting in a minimum detectable CO production of 20 ppb in a 1-m optical path above a standard cell-culture flask. Data-processing procedures for real-time monitoring of both biogenic and ambient atmospheric CO concentrations are described.