, Volume 106, Issue 2, pp 339-348
Date: 11 Nov 2011

Optimized coherence parameters for high-resolution holographic microscopy

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Abstract

A holographic in-line microscope setup with a glass sample carrier commonly uses a coherent laser light source, with the disadvantage of an incomplete suppression of disturbing interferences and coherence-induced noise.

We use temporally partially coherent and spatially partially coherent illumination in the micrometer range of 5 to 100 μm generated by spectrally and spatially filtered light-emitting diodes (LEDs) and tungsten filament lamps. It is shown how errors in reconstructed holograms are decreased by a factor of more than two and the spatial resolution reaches the limit as determined by the numerical aperture. A straightforward model is developed to investigate the effects of partial coherence. The optimization of the coherence parameters improves the imaging quality up to the limit of a conventional microscope.