Applied Physics B

, Volume 106, Issue 2, pp 339–348

Optimized coherence parameters for high-resolution holographic microscopy

Article

DOI: 10.1007/s00340-011-4787-6

Cite this article as:
Petruck, P., Riesenberg, R. & Kowarschik, R. Appl. Phys. B (2012) 106: 339. doi:10.1007/s00340-011-4787-6

Abstract

A holographic in-line microscope setup with a glass sample carrier commonly uses a coherent laser light source, with the disadvantage of an incomplete suppression of disturbing interferences and coherence-induced noise.

We use temporally partially coherent and spatially partially coherent illumination in the micrometer range of 5 to 100 μm generated by spectrally and spatially filtered light-emitting diodes (LEDs) and tungsten filament lamps. It is shown how errors in reconstructed holograms are decreased by a factor of more than two and the spatial resolution reaches the limit as determined by the numerical aperture. A straightforward model is developed to investigate the effects of partial coherence. The optimization of the coherence parameters improves the imaging quality up to the limit of a conventional microscope.

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  1. 1.Institute of Photonic Technology (IPHT)JenaGermany
  2. 2.Institute of Applied OpticsUniversity of JenaJenaGermany