Applied Physics B

, Volume 99, Issue 4, pp 633–637

High-speed spectrally resolved multifocal multiphoton microscopy

Authors

  • Y. Shao
    • Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province Institute of OptoelectronicsShenzhen University
    • Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province Institute of OptoelectronicsShenzhen University
  • H. Li
    • Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province Institute of OptoelectronicsShenzhen University
  • Y. Wang
    • Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province Institute of OptoelectronicsShenzhen University
  • J. Qi
    • Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province Institute of OptoelectronicsShenzhen University
  • G. Xu
    • Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province Institute of OptoelectronicsShenzhen University
  • H. Niu
    • Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province Institute of OptoelectronicsShenzhen University
Article

DOI: 10.1007/s00340-010-4066-y

Cite this article as:
Shao, Y., Qu, J., Li, H. et al. Appl. Phys. B (2010) 99: 633. doi:10.1007/s00340-010-4066-y

Abstract

We present a spectrally resolved multifocal multiphoton microscopy that is capable of performing fast 2-dimensional (2-D) spectral measurements of fluorescent samples with optical sectioning. One galvanometer mirror is used to scan the array of excitation foci across the sample along one direction (x) for two-photon excitation. Fluorescence emission from the excited lines on the sample is spectrally fanned out with a prism along the y direction, and a CCD array is used to acquire the spectrally resolved image. Another galvanometer mirror scans the excitation foci lines along the y direction step by step to obtain 3-dimensional (3-D) spectral data cube of the sample. A proof-of-principle experiment is performed with fluorescent microspheres of different colors. Spectrally resolved images of 512×512 pixels can be obtained by acquiring only 128 raw images when a 4×4 excitation foci array is used.

Copyright information

© Springer-Verlag 2010