Applied Physics B

, Volume 87, Issue 4, pp 629–634

Tunable mid-infrared, high-energy femtosecond laser source for glyco-protein structure analysis

Authors

  • T. Imahoko
    • Cyber Laser Inc.
    • School of Integrated Design EngineeringKeio University
  • K. Takasago
    • Cyber Laser Inc.
  • T. Sumiyoshi
    • Cyber Laser Inc.
  • H. Sekita
    • Cyber Laser Inc.
  • K. Takahashi
    • Computational Biology Research CenterAIST Tokyo Waterfront
    • School of Integrated Design EngineeringKeio University
Article

DOI: 10.1007/s00340-007-2657-z

Cite this article as:
Imahoko, T., Takasago, K., Sumiyoshi, T. et al. Appl. Phys. B (2007) 87: 629. doi:10.1007/s00340-007-2657-z

Abstract

We have developed a 6–12 μm mid-infrared (MIR) femtosecond laser source for glyco-protein structure analysis. The MIR femtosecond laser pulses are generated by a differential frequency generation (DFG) configuration with a combination of Ti:sapphire based regeneratively amplified femtosecond laser pulses (780 nm, 160 fs, 1 mJ) and a β-BaB2O4 (BBO) based optical parametric amplifier (OPA). The MIR pulse energy exceeds 4.5 μJ, where a glyco-protein molecule has resonant absorption lines due to the vibrational–rotational transitions. The pulse width is estimated to be less than 1 ps according to the cross correlation measurement between the two OPA output pulses. Using the MIR femtosecond laser pulses, we demonstrated photo-dissociation of the sialyl Lewis X (sLeX) proton added ion, which is the first time to the best of our knowledge.

Copyright information

© Springer-Verlag 2007