Applied Physics B

, Volume 87, Issue 3, pp 389–393

Femtosecond stimulated Raman microscopy

Authors

  • E. Ploetz
    • Lehrstuhl für BioMolekulare Optik and CIPSM, Department für PhysikLudwig-Maximilians-Universität
  • S. Laimgruber
    • Lehrstuhl für BioMolekulare Optik and CIPSM, Department für PhysikLudwig-Maximilians-Universität
  • S. Berner
    • Lehrstuhl für BioMolekulare Optik and CIPSM, Department für PhysikLudwig-Maximilians-Universität
  • W. Zinth
    • Lehrstuhl für BioMolekulare Optik and CIPSM, Department für PhysikLudwig-Maximilians-Universität
    • Lehrstuhl für BioMolekulare Optik and CIPSM, Department für PhysikLudwig-Maximilians-Universität
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DOI: 10.1007/s00340-007-2630-x

Cite this article as:
Ploetz, E., Laimgruber, S., Berner, S. et al. Appl. Phys. B (2007) 87: 389. doi:10.1007/s00340-007-2630-x

Abstract

A novel type of non-linear Raman microscopy, femtosecond stimulated Raman microscopy (FSRM), is introduced. It employs femtosecond white light pulses and intense picosecond pulses which are derived from a femtosecond laser/amplifier system. The pulses are coupled into a microscope set-up and induce a stimulated Raman process at the focus. The Raman interaction spectrally modulates the white light. These modulations are read-out in multi-channel fashion and allow recording of a complete Raman spectrum of the focal region. By raster-scanning the sample, complete Raman images can be obtained. Raman images of polystyrene beads in water demonstrate the feasibility of the approach.

Copyright information

© Springer-Verlag 2007