, Volume 75, Issue 2-3, pp 397-403

Simultaneous online detection of isoprene and isoprene-d2 using infrared photoacoustic spectroscopy

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A photoacoustic spectrometer for the simultaneous detection of isoprene and the deuterated species [4,4-2H]-2-methyl-1,3-butadiene (isoprene-d2) is presented. Using a sealed-off 13CO2 laser a single-component detection limit of 400 ppt (isoprene) and 600 ppt (isoprene-d2) was achieved. Simultaneous monitoring of both compounds allowed the detection of labelling levels down to 6% (isoprene-d2 in total isoprene) with a time resolution of 3 min.

In emission studies with Eucalyptus globulus, the deuterated precursor [4,4-2H]-1-deoxy-D-xylulose was fed to a leaf through the transpiration stream. Emission of isoprene-d2 started as early as 10 min after application of the precursor.

Received: 3 May 2002 / Revised version: 31 May 2002 / Published online: 21 August 2002
ID="*"Corresponding author. Fax: +49-228/733474, E-mail: frank.kuehnemann@iap.uni-bonn.de