Applied Physics B

, Volume 74, Supplement 1, pp s97–s101

Third-harmonic microscopy with a titanium–sapphire laser

  • D. Yelin
  • D. Oron
  • E. Korkotian
  • M. Segal
  • Y. Silberberg
Article

DOI: 10.1007/s00340-002-0884-x

Cite this article as:
Yelin, D., Oron, D., Korkotian, E. et al. Appl Phys B (2002) 74: s97. doi:10.1007/s00340-002-0884-x

Abstract.

We demonstrate third-harmonic microscopy of thin biological objects using a Ti:sapphire laser source. A very simple modification to the collection and detection systems of the microscope allows for the detection of the third-harmonic signal near 270 nm. We show that, using a Ti:sapphire laser, we can combine third-harmonic imaging with two-photon excitation fluorescence simultaneously. Compared to other possible laser sources for third-harmonic microscopy, the Ti:sapphire laser provides better optical resolution, better power availability, and is less absorbed in water. We find that the Ti:sapphire laser is the most suitable laser source for third-harmonic microscopy for thin biological specimens.

PACS: 42.65.Ky; 87.64.Vv; 42.62.Be

Copyright information

© Springer-Verlag 2002

Authors and Affiliations

  • D. Yelin
    • 1
  • D. Oron
    • 1
  • E. Korkotian
    • 2
  • M. Segal
    • 2
  • Y. Silberberg
    • 1
  1. 1.Department of Physics of Complex Systems, The Weizmann Institute of Science, Rehovot 76100, IsraelIL
  2. 2.Department of Neural Biology, The Weizmann Institute of Science, Rehovot 76100, IsraelIL