Calcification by juvenile corals under heterotrophy and elevated CO2
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- Drenkard, E.J., Cohen, A.L., McCorkle, D.C. et al. Coral Reefs (2013) 32: 727. doi:10.1007/s00338-013-1021-5
Ocean acidification (OA) threatens the existence of coral reefs by slowing the rate of calcium carbonate (CaCO3) production of framework-building corals thus reducing the amount of CaCO3 the reef can produce to counteract natural dissolution. Some evidence exists to suggest that elevated levels of dissolved inorganic nutrients can reduce the impact of OA on coral calcification. Here, we investigated the potential for enhanced energetic status of juvenile corals, achieved via heterotrophic feeding, to modulate the negative impact of OA on calcification. Larvae of the common Atlantic golf ball coral, Favia fragum, were collected and reared for 3 weeks under ambient (421 μatm) or significantly elevated (1,311 μatm) CO2 conditions. The metamorphosed, zooxanthellate spat were either fed brine shrimp (i.e., received nutrition from photosynthesis plus heterotrophy) or not fed (i.e., primarily autotrophic). Regardless of CO2 condition, the skeletons of fed corals exhibited accelerated development of septal cycles and were larger than those of unfed corals. At each CO2 level, fed corals accreted more CaCO3 than unfed corals, and fed corals reared under 1,311 μatm CO2 accreted as much CaCO3 as unfed corals reared under ambient CO2. However, feeding did not alter the sensitivity of calcification to increased CO2; ∆ calcification/∆Ω was comparable for fed and unfed corals. Our results suggest that calcification rates of nutritionally replete juvenile corals will decline as OA intensifies over the course of this century. Critically, however, such corals could maintain higher rates of skeletal growth and CaCO3 production under OA than those in nutritionally limited environments.
KeywordsClimate changeOcean acidificationCoral reefsCoral calcificationHeterotrophyEnergetics
The ocean has absorbed 25–30 % of the CO2 emitted by human activities, driving a 0.1 unit decline in surface ocean pH and a 30 % decrease in carbonate ion concentration ([CO32−]), a process known as ocean acidification (OA) (Caldeira and Wickett 2003; Feely et al. 2004). Scleractinian corals build skeletons of aragonite, a polymorph of calcium carbonate (CaCO3), and rely on carbonate ions for calcification (Marubini and Atkinson 1999; Silverman et al. 2007; de Putron et al. 2011). The aragonite saturation state (Ωar, [Ca2+][CO32−]/Ksp(arag)) of seawater reflects the thermodynamic tendency for CaCO3 to form (Ω >1) or dissolve (Ω <1). Although the tropical oceans where most coral reefs are located are not likely to become under saturated with respect to aragonite (Ωar <1) during this century, most experimental studies show that skeletal growth and CaCO3 production by corals are negatively impacted by OA long before aragonite under saturation is reached. On the ecosystem scale, the relative rates of CaCO3 production versus dissolution are critical for coral reefs. If rates of production fall below natural rates of erosion and dissolution, reefs will shift from net accreting to net dissolving structures (Orr et al. 2005; Silverman et al. 2009), diminishing their capacity to provide habitats for marine organisms and to function as effective barriers against waves and tsunamis. Using coral reef community calcification data from the Gulf of Aqaba, Silverman et al. (2009) predicted a global-scale shift from net accreting to net dissolving reefs within the next 60 yrs.
The impact of Ωar on calcification by reef organisms has been explored largely in laboratory manipulation experiments, although a handful of in situ datasets provide key insights into the sensitivity of ecosystem-scale calcification to rising CO2 levels (e.g., Silverman et al. 2007; Shamberger et al. 2011). In general, these studies have shown that both coral and coral reef calcification decline with decreasing Ωar (reviewed in Langdon et al. 2000; Hoegh-Guldberg et al. 2007; Fabry et al. 2008; Doney et al. 2009; Pandolfi et al. 2011), but there is considerable variability among populations, species, and studies in the calcification response or sensitivity at a given Ωar (summarized in Pandolfi et al. 2011). There is also variability in the absolute rates of calcification among different corals and coral reefs at the same Ωar (Shamberger et al. 2011). For example, flume incubations of Hawaiian Porites compressa and Montipora verucosa show a positive, linear relationship between Ωar and calcification rate (mmol CaCO3 m−2 h−1) (Langdon and Atkinson 2005). However, de Putron et al. (2011) reported a nonlinear relationship between Ωar and calcification by Bermudan Favia fragum and Porites asteroides over a similar range of Ωar, while Ries et al. (2010) found that Oculina sp. responded only at a treatment pCO2 of 2,800 ppm (Ωar <1) and not at values of 900 ppm and below. On the scale of coral reef communities, different reef ecosystems at the same Ωar exhibit significant differences in the rate of net reef calcification. For example, the average net calcification rate of a Red Sea reef was reported as 54.5 mmol CaCO3 m−2 h−1 at an average Ωar of 3.9 (Silverman et al. 2007), whereas average net calcification rate of the Kaneohe Bay barrier reef on Hawaii was significantly higher (264.2 mmol CaCO3 m−2 h−1) despite a significantly lower average Ωar (2.9) (Shamberger et al. 2011). Multiple environmental and biological factors that influence biogenic calcification on a coral reef could be invoked to explain the variability, but few have been directly tested.
Here, we conducted an experiment in which the nutritional status of zooxanthellate (photosynthesizing) juvenile corals, that were reared under very high and ambient pCO2, was enhanced via heterotrophic feeding. A number of experimental and field studies have demonstrated (Langdon and Atkinson 2005; Holcomb et al. 2010) or suggested (Atkinson et al. 1995; Atkinson and Cuet 2008; Cohen and Holcomb 2009; Shamberger et al. 2011; Edmunds 2011) that elevated dissolved inorganic nutrients (DIN) and/or nutrition via heterotrophic feeding could reduce the impact of elevated CO2 on calcification. We chose to manipulate heterotrophic feeding conditions because the addition of DIN to coral cultures under ambient CO2 can lead to decreased calcification due to a proposed disruption in the coral-zooxanthellae symbiosis (Muscatine et al. 1989; Falkowski et al. 1993; Marubini and Davis 1996), whereas heterotrophic feeding tends to enhance calcification under ambient CO2 conditions (Houlbrèque and Ferrier-Pagès 2009).
Materials and methods
Experimental setup and conditions
Measurements and calculations of experimental carbonate and seawater chemistry conditions
Salinity (psu ± SD)
Alkalinity (μeq kg−1 ± SD)
DIC (μmol kg−1 ± SD)
pCO2 (μatm ± SD)
pH (total ± SD)
[HCO3−] (μmol kg−1 ± SD)
[CO32−] (μmol kg−1 ± SD)
Ambient CO2, fed
37.6 ± 0.3
2,332 ± 22
2,012 ± 33
443 ± 40
8.00 ± 0.03
1,775 ± 40
225 ± 9
3.55 ± 0.16
Ambient CO2, unfed
37.4 ± 0.3
2,325 ± 20
1,984 ± 16
398 ± 4
8.04 ± 0.00
1,735 ± 13
239 ± 3
3.77 ± 0.03
High CO2, fed
37.0 ± 0.2
2,324 ± 9
2,213 ± 16
1,344 ± 78
7.59 ± 0.02
2,077 ± 17
100 ± 4
1.59 ± 0.06
High CO2, unfed
37.0 ± 0.2
2,326 ± 23
2,207 ± 21
1,278 ± 70
7.61 ± 0.02
2,069 ± 20
105 ± 5
1.66 ± 0.08
Each CO2-feeding treatment was conducted in triplicate for a total of twelve aquaria, and all treatments were kept on a 12/12 h light–dark cycle. Fluorescent aquarium lamps maintained maximum light levels of 62 ± 8 μmol quanta m−2 s−1 (mean ± SD), which were monitored using a LI-COR probe/meter assemblage. The compensation range for F. fragum spat on Bermuda is not yet known. We used the low end of known compensation ranges for corals (e.g. 3–233 μmol quanta m−2 s−1 as reported by Mass et al. 2007) for two reasons. The first was to ensure that corals under elevated CO2 did not bleach (as experienced by Anthony et al. 2009), and the second was to minimize the potential for enhanced photosynthesis to overwhelm or inhibit the feeding-modulated calcification response to elevated CO2. Aquarium temperatures were maintained by in-line chiller/heater systems and monitored every 15 min (Hobo temperature loggers, Onset Corp.). Average temperature for all treatments over the course of the experiment was 27.6 ± 0.1 °C (± SD).
Aquarium water was replaced with filtered seawater every week to prevent the build-up of dissolved inorganic nitrogen and other wastes. Prior to removing water from the aquaria, we collected discrete water samples for salinity, alkalinity (Alk), and dissolved inorganic carbon (DIC) from every aquarium. Salinity was measured at BIOS with an Autosal salinometer. The Alk/DIC samples were poisoned with mercuric chloride immediately after collection and analyzed using a Marianda VINDTA-3C analysis system at WHOI. Alkalinity was determined by nonlinear curve fitting of data obtained by open-cell titrations, and DIC concentrations were determined by coulometric analysis. Both measurements were standardized using certified reference materials obtained from Dr. A. Dickson (Scripps IO). The pH (NBS) of each tank was measured every 3–4 d (Orion pH meter and temperature-compensated electrode) to provide a real-time assessment of tank chemistry. Short-term variations in NBS pH were also assessed on a higher-resolution time scale: for one, 24-h period, by measuring pH in each aquarium at 3-h time intervals. The pH within each tank was maintained within ± a few hundredths of a pH unit on both sub-weekly and sub-daily time scales. The carbonate system parameters used to compare treatments (pCO2, [HCO3−], [CO32−], and Ωar) were calculated from the average temperature and discretely sampled salinity, Alk, and DIC data using the CO2SYS program (Lewis and Wallace 1998; Pelletier et al. 2007) with the constants of Mehrbach et al. (1973) as refit by Dickson and Millero (1987) (Table 1).
Coral collection, spawning, and larval settlement
In July 2010, approximately 1 week prior to anticipated peak larval release date (Goodbody-Gringley and de Putron 2009), we collected 30 mature colonies of the brooding coral, F. fragum, from the Bailey’s Bay patch reefs off the northwest Bermudan coast at approximately three to seven meters water depth. Adult colonies were maintained in outdoor flow-through seawater aquaria at BIOS under ambient light and temperature conditions. Parent colonies were kept isolated in glass jars during planula release, which occurred over the course of 6 nights. The live zooxanthellate planulae were collected from all parents and pooled together. Ceramic tiles, approximately 9 cm2, were left out on the reef for 2 months prior to the start of the experiment and further conditioned for larval settlement by scattering bits of freshly collected crustose coralline algae on the tiles. Immediately after collection, actively swimming larvae were transferred to small plastic tubs each containing ceramic tiles and filled with seawater preset to targeted CO2 levels. The tubs had mesh lids, allowing for water exchange, while they are submerged in the treatment aquaria. After 48 h, larvae had settled and metamorphosed into primary polyps (at this stage, larvae are “spat”). Spat on tiles were quickly counted, and tiles were pseudo-randomly distributed among the experimental aquaria so that each aquarium had approximately the same number of juvenile corals. Calcification was visible approximately 3 d after settlement. At the end of 3 weeks (±1 d), 20–50 primary polyps (including their primary corallite) per treatment were removed from the tiles and frozen at −80 °C for analysis of total lipid. Tiles were then removed from treatments and submerged in a 10 % bleach solution for 1 h, which removed the polyp tissue from all of the remaining juvenile corals and exposed the calcified skeleton or primary corallite.
Quantification of skeletal development, size, and weight
To test for differences in mean spat weight and diameter among treatments, a two-way, nested multivariate analysis of variance (MANOVA) was performed on natural log transformed weight data and square root transformed diameter data. Feeding treatment and CO2 levels were fixed main effects, while tank effect was the random factor nested within feeding and CO2 levels (ESM Table S2). Eight univariate F tests were conducted to test each of the dependent variables. A Bonferonni corrected alpha value of 0.0062 was used to declare significance of F statistics (ESM Table S3). It should be noted that the MANOVA only considers corals that have data for both diameter and weight. If part of a corallite is lost during weighing or was attached to coralline algae, both coral size and weight were excluded from the MANOVA analyses. Likewise, if the skeleton was irregularly shaped (i.e., primary septa did not lie in a straight line), the data for those corals were not included. In order to account for any bias that may have resulted from corallite exclusion in the MANOVA, ANOVAs for the dependent variables, weight, and diameter were conducted. These tests considered all data for a given dependent variable to compare with the MANOVA’s univariate results.
Quantification of total lipid and symbiont density
Ten individual spat from each aquarium were pooled per tissue lipid sample for quantification of total lipid by gravimetric analysis. Pooling was necessary due to the small size of the spat at 3 weeks. Extraction methods follow that of Folch et al. (1957) and Cantin et al. (2007).
Five individual spat from each aquarium were pooled per sample for quantification of symbiont density. Spat were homogenized, centrifuged and the resultant pellet was re-suspended in 250 μL filtered seawater. Symbionts from multiple (6–9) aliquot sub-samples of the slurry were counted on a known volume hemocytometer grid. Both total tissue lipid and symbiont counts were normalized to the circular area described by the average primary septa length (diameter) for a respective tank and then divided by the number of corals pooled in the sample (i.e., 10 or 5).
Both area-normalized lipid content and symbiont density were compared among levels of CO2 and feeding conditions using two-way ANOVAs with tank as a random factor nested within the CO2 and feeding combinations. Total lipid concentration was transformed to −1/x in order to homogenize the variances. All statistical analyses were conducted on SYSTAT.
Skeletal size and weight
Lipid and symbiont density
Skeletal size and development, rate of CaCO3 production, and energetic status (e.g., total lipid stores and metabolic performance) are key physiological indices of coral health and fitness. High growth rate contributes to juvenile coral survival and successful reef recruitment (Rylaarsdam 1983; Hughes and Jackson 1985; Vermeij and Sandin 2008). Linear extension affects a colony’s ability to compete for space with algae and reduced skeletal density may affect the structural integrity of the coral holobiont (Hoegh-Guldberg et al. 2007). Further, energetic reserves have been used to model and predict coral colony mortality risk (Anthony et al. 2009). These parameters are sensitive to a number of environmental stressors. For example, skeletal growth and calcification tend to decline in corals stressed by elevated temperatures (e.g., Rodrigues and Grottoli 2006; Cooper et al. 2008; Cantin et al. 2010) or eutrophication (Marubini and Atkinson 1999), and bleaching can result in rapid depletion of energetic reserves (e.g., Grottoli et al. 2004; Rodrigues and Grottoli 2007). Given anticipated (Kleypas et al. 1999) and experimentally observed (e.g., Langdon and Atkinson 2005) declines in coral calcification due to acidification, it has been suggested that OA may increase the energetic demands of CaCO3 production (Cohen and Holcomb 2009; Holcomb et al. 2010; Ries 2011).
In this study, OA induced by significantly elevated levels of CO2 had no effect on the rate of development of septal cycles and skeletal diameter (size) nor could we detect a significant effect on area-normalized total tissue lipid content and symbiont density of juvenile corals reared from planulae larvae (Figs. 2, 4). Conversely, fed juveniles reared under elevated CO2 conditions (~5 times preindustrial) exhibited faster tertiary septa development and had larger skeletons than unfed juveniles reared under ambient CO2 levels (~1.5 times preindustrial). Thus, for newly settled corals of this species, OA may have little, if any, impact on lateral size and septal development, whereas factors that impact food availability or a coral’s ability to acquire food could affect these aspects of post-settlement growth.
Heterotrophic feeding also significantly impacted the rate of CaCO3 production (as measured by total corallite weight). Under both ambient and elevated CO2 conditions, fed corals produced significantly more CaCO3 over the 3-week experimental period than unfed corals (Fig. 2c). At 421 μatm CO2, fed corals produced 55 % more CaCO3 than unfed corals; at 1,311 μatm CO2, the difference was 68 %. Thus, under significantly elevated CO2 conditions, fed spat develop faster, grow bigger, and weigh more than unfed spat. This suggests that, to the extent that young corals affect the reef CaCO3 budget, nutritionally enhanced juveniles contribute more CaCO3 than those that are nutritionally restricted and subjected to the same CO2 conditions. Remarkably, fed juveniles subjected to significantly elevated CO2 also develop faster and grow larger than unfed corals reared under ambient CO2 conditions, and their rate of CaCO3 production are comparable. Therefore, by implication, nutritionally replete corals could perform better under OA than corals that are nutritionally restricted.
Nevertheless, our results indicate that feeding does not mitigate the impact of OA on calcification by juvenile corals. In both fed and unfed groups, skeletal weight decreased, by 23.0 ± 2.9 and 28.9 ± 0.1 %, respectively (~8–14 % per unit drop in omega), under elevated CO2. This change is equivalent to that observed by de Putron et al. (2011) for both acid addition and CO2 manipulation experiments with the same Favia species, although it is significantly less than the 80 % drop predicted by the Langdon and Atkinson model (2005).
A number of studies report increased calcification by corals under heterotrophic feeding (e.g., Houlbrèque and Ferrier-Pagès 2009), which is consistent with the observations in this study. However, our data show that the negative effect of OA on calcification persists under conditions of heterotrophic feeding. In our study, feeding did not change the sensitivity of calcification to OA. This suggests that nutritional enhancement via heterotrophic feeding did not change the mechanics of the calcification response to OA in our corals. Although Edmunds (2011) concluded that heterotrophic feeding does mitigate the impact of elevated CO2 on juvenile Porites calcification, both the fed and unfed Porites corals in his experiment exhibited reduced biomass-corrected calcification under elevated CO2, which is consistent with our result for Favia. Indeed, Edmunds’ (2011) result lends support to our observation that the sensitivity of calcification response to elevated CO2 is consistent between fed and unfed corals. In other words, heterotrophic feeding does not mitigate the effect of OA on coral calcification.
That heterotrophic feeding does not mitigate the impact of OA on juvenile coral differs from the results of Langdon and Atkinson (2005) and Holcomb et al. (2010) who reported significant modulation of the CO2 effect with inorganic nutrient enrichment. In these studies, addition of ammonium, and of nitrates, phosphates, and iron, respectively, did reduce calcification sensitivity to OA. In the experiments of Langdon and Atkinson (2005), nutrient addition enhanced symbiont photosynthesis (photosynthesis was not measured in Holcomb et al. (2010)). We were not able to detect a significant impact on area-normalized symbiont densities due to feeding in our experiments (Fig. 4b). This observation is different from that reported by a number of previous studies (Muscatine et al. 1989; Titlyanov et al. 2000a, b, 2001; Houlbrèque et al. 2003, 2004) and may be due to our lack of statistical power to detect a significant feeding effect. Alternatively, although our corals were fully zooxanthellate at the time of settlement, the impact of feeding on symbiont densities might differ between young corals and the mature colonies used in other experiments. Endosymbiont density is only one component of the coral holobiont’s photosynthetic capacity and is not a substitute for direct measurements of photosynthesis (e.g., Langdon and Atkinson 2005) because the performance of individual symbionts is still unknown. Therefore, we can only speculate that the difference between our result (i.e., no significant difference detected in symbiont density or reduction in sensitivity to CO2 due to feeding) and that of Langdon and Atkinson (2005), that is, DIN enrichment resulting in enhanced photosynthesis and reduced sensitivity to CO2, suggest a role for symbiont photosynthesis in the coral calcification response to OA. From our data, it does not appear that simply enhancing coral energetic status (in this case, via feeding) alters calcification sensitivity to OA. However, photosynthesis and heterotrophy may impact coral calcification via different mechanisms. If this is the case, then the impact of OA on calcification when photosynthesis is enhanced might differ from the impact of OA on calcification when feeding is enhanced. Incidentally, it should be noted that the degree to which feeding impacted calcification rates in this study may be specific to our relatively low-light regime and test species and could therefore differ among organisms subjected to higher light environments.
Additionally, fast-growing, prereproductive juvenile corals might respond to feeding differently from adults, which were used in both the Langdon and Atkinson (2005) and Holcomb et al. (2010) experiments. Adult corals may allocate the extra energy from heterotrophic feeding differently from juveniles. To investigate whether our juvenile corals were storing the extra energy from heterotrophic feeding as lipid reserve, or using it to build new tissue or skeleton, we averaged total tissue lipid content over circular surface area (Fig. 4b). We could not detect a significant difference in the amount of lipid accumulated by the corals in the different feeding regimes. This suggests that, in this particular experiment, the fed coral spat did not store the extra energy acquired from feeding but rather used it for growth. Whether or not mature colonies in experimental conditions and on actual reefs respond to food availability the same way, that is, by investing in tissue growth rather than lipid storage, is yet to be tested.
Our results show that healthy, nutritionally replete spat of the Atlantic coral, F. fragum, can sustain high rates of calcification under significantly elevated CO2. However, enhanced nutritional status does not render these corals immune to OA. This has important implications for the ability of corals and coral reefs to maintain levels of growth and CaCO3 production required to sustain reef ecosystems through increasingly hostile conditions over the twenty-first century.
The authors are grateful to Rebecca Belastock (WHOI) for DIC/Alk analyses. We also thank Ms. Hannah Barkley (WHOI), Ms. Kascia White (BIOS) and Mr. Mark Dowar (BIOS) for assistance with fieldwork and experiment maintenance, and Dr Neal Cantin (WHOI) for assistance with laboratory procedures. This project was funded by NSF OCE-1041106 and NSF OCE-1041052, a WHOI winter intern fellowship to A. Zicht made possible by the A. V. Davis Foundation and support from the MIT/WHOI Bermuda Biological Station for Research Fund. We thank the three anonymous reviewers and the editor for suggestions that greatly improved the manuscript.