Mammalian Genome

, Volume 10, Issue 12, pp 1160–1167

Alternative splicing, gene localization, and binding of SH2-B to the insulin receptor kinase domain

  • Keats  Nelms
  • Thomas J.  O'Neill
  • Shiqing  Li
  • Stevan R.  Hubbard
  • Thomas A.  Gustafson
  • William E.  Paul
Article

DOI: 10.1007/s003359901183

Cite this article as:
Nelms, K., O'Neill, T., Li, S. et al. Mammalian Genome (1999) 10: 1160. doi:10.1007/s003359901183

Abstract.

The SH2-B protein is an SH2-domain-containing molecule that interacts with a number of phosphorylated kinase and receptor molecules including the insulin receptor. Two isoforms of the SH2-B have been identified and have been proposed to arise through alternate splicing. Here we have identified a third isoform of the SH2-B protein, SH2-Bγ, that interacts specifically with the insulin receptor. This interaction required phosphorylation of residue Y1146 in the triple tyrosine motif within the activation loop of the IR kinase and is one of only two signaling molecules shown to interact directly with this residue of the insulin receptor kinase domain. The intron/exon structure of the SH2-B gene was determined. Alternate splice sites utilized to generate the different isoforms of the SH2-B protein were identified in the 3′ end of the SH2-B gene immediately downstream of the exon encoding the core of the SH2 domain. Additionally, the chromosomal location of the SH2-B gene was determined to be the distal arm of mouse Chromosome (Chr) 7 in a region linked to obesity in mice.

Copyright information

© Springer-Verlag New York Inc. 1999

Authors and Affiliations

  • Keats  Nelms
    • 1
  • Thomas J.  O'Neill
    • 2
  • Shiqing  Li
    • 3
  • Stevan R.  Hubbard
    • 3
  • Thomas A.  Gustafson
    • 2
  • William E.  Paul
    • 1
  1. 1.Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USAUS
  2. 2.Department of Physiology, University of Maryland School of Medicine, Baltimore, Maryland 21201, USAUS
  3. 3.Skirball Institute of Biomolecular Medicine and Department of Pharmacology, New York University Medical Center, 540 First Avenue, New York, New York 10016, USAUS
  4. 4.Metabolex, Inc., 3876 Bay Center Place, Hayward, California 94545, USAUS

Personalised recommendations