Mammalian Genome

, Volume 10, Issue 6, pp 585–587

Construction and characterization of a sheep BAC library of three genome equivalents

  • Daniel Vaiman
  • Alain Billault
  • Kamila Tabet-Aoul
  • Laurent Schibler
  • Didier Vilette
  • Anne Oustry-Vaiman
  • Catherine Soravito
  • Edmond P. Cribiu
Article

DOI: 10.1007/s003359901049

Cite this article as:
Vaiman, D., Billault, A., Tabet-Aoul, K. et al. Mammalian Genome (1999) 10: 585. doi:10.1007/s003359901049

Abstract

A sheep BAC library of over three genome equivalents was constructed and arrayed in superpools and row, column, and plate pools. The library contains 90,000 clones distributed in 39 superpools. The average insert size was estimated at 123 kb. The library was screened by PCR with 77 primer pairs corresponding to ovine microsatellites distributed throughout the genome. The probability of finding a random sequence in the library could be estimated at 0.96.

Copyright information

© Springer-Verlag New York Inc. 1999

Authors and Affiliations

  • Daniel Vaiman
    • 1
  • Alain Billault
    • 2
  • Kamila Tabet-Aoul
    • 3
  • Laurent Schibler
    • 1
  • Didier Vilette
    • 1
  • Anne Oustry-Vaiman
    • 1
  • Catherine Soravito
    • 2
  • Edmond P. Cribiu
    • 1
  1. 1.Laboratoire de Génétique biochimique et de Cytogénétique, Département de Génétique Animale, INRA, 78352 Jouy-en-Josas, FranceFR
  2. 2.Fondation Jean Dausset-Centre d'Etude du Polymorpisme Humain, 27, rue Juliette Dodu, 75010 Paris, FranceFR
  3. 3.Laboratoire de Pathologie infectieuse et d'Immunologie, INRA, 37380, Nouzilly, FranceFR