Mammalian Genome

, Volume 10, Issue 3, pp 266–270

Molecular cloning and chromosomal assignment of the porcine 54 and 56 kDa vacuolar H(+)-ATPase subunit gene (V-ATPase)

  • Dequan  Hui
  • Alexandra  Deppe
  • Gaiping  Wen
  • Tosso  Leeb
  • Julio  Masabanda
  • Annie  Robic
  • Bernhard G.  Baumgartner
  • Ruedi  Fries
  • Bertram  Brenig

DOI: 10.1007/s003359900984

Cite this article as:
Hui, D., Deppe, A., Wen, G. et al. (1999) 10: 266. doi:10.1007/s003359900984

Abstract.

Vacuolar proton-translocating ATPases (V-ATPase) are multisubunit enzyme complexes located in the membranes of eukaryotic cells regulating cytoplasmic pH. So far, nothing is known about the genomic organization and chromosomal location of the various subunit genes in higher eukaryotes. Here we describe the isolation and analysis of a cDNA coding for the 54- and 56-kDa porcine V-ATPase subunit alpha and beta isoforms. We have determined the genomic structure of the V-ATPase subunit gene spanning at least 62 kb on Chromosome (Chr) 4q14-q16. It consists of 14 exons with sizes ranging from 54 bp to 346 bp, with a non-coding first exon and an alternatively spliced seventh exon leading to two isoforms. The 5′ end of the V-ATPase cDNA was isolated by RACE-PCR. The V-ATPase alpha isoform mRNA, lacking the seventh exon, has an open reading frame of 1395 nucleotides encoding a hydrophilic protein of 465 amino acids with a calculated molecular mass of 54.2 kDa and a pI of 7.8, whereas the beta isoform has a length of 1449 nucleotides encoding a protein of 483 amino acids with a calculated molecular mass of 55.8 kDa. Amino acid and DNA sequence comparison revealed that the porcine V-ATPase subunit exhibits a significant homology to the VMA13 subunit of Saccharomyces cerevisiae V-ATPase complex and V-ATPase subunit of Caenorhabditis elegans.

Copyright information

© Springer-Verlag New York Inc. 1999

Authors and Affiliations

  • Dequan  Hui
    • 1
  • Alexandra  Deppe
    • 1
  • Gaiping  Wen
    • 1
  • Tosso  Leeb
    • 1
  • Julio  Masabanda
    • 2
  • Annie  Robic
    • 3
  • Bernhard G.  Baumgartner
    • 1
  • Ruedi  Fries
    • 2
  • Bertram  Brenig
    • 1
  1. 1.Institute of Veterinary Medicine, University of Göttingen, Groner Landstrasse 2, 37073 Göttingen, GermanyDE
  2. 2.Institute of Animal Breeding and Genetics, Technical University of Munich, Weihenstephan, GermanyDE
  3. 3.INRA, Centre de Recherches de Toulouse, Laboratoire de Génétique Cellulaire, FranceFR