Mammalian Genome

, Volume 10, Issue 2, pp 81–87

Identification of peak bone mass QTL in a spontaneously osteoporotic mouse strain

  • Motoyuki  Shimizu
  • Keiichi  Higuchi
  • Beth  Bennett
  • Chen  Xia
  • Tadao  Tsuboyama
  • Soichiro  Kasai
  • Takuya  Chiba
  • Hiromi  Fujisawa
  • Kumiko  Kogishi
  • Haruo  Kitado
  • Mitsutoshi  Kimoto
  • Norikazu  Takeda
  • Mutsumi  Matsushita
  • Hideo  Okumura
  • Tadao  Serikawa
  • Takashi  Nakamura
  • Thomas E.  Johnson
  • Masanori  Hosokawa

DOI: 10.1007/s003359900949

Cite this article as:
Shimizu, M., Higuchi, K., Bennett, B. et al. (1999) 10: 81. doi:10.1007/s003359900949

Abstract.

The whole genome scan for quantitative trait loci (QTLs) specifying peak bone mass was performed with the F2 intercrosses of SAMP6, an established murine model of senile osteoporosis, exhibiting a significantly lower peak bone mass, and SAMP2, exhibiting a higher peak bone mass. Cortical thickness index (CTI), a parameter of bone mass of femurs, was measured in 488 F2 progeny at 4 months of age, when the animals attained peak bone mass by microphotodensitometry. Genetic markers were typed at 90 loci spanning all chromosomes except the Y. By interval mapping of 246 male F2 mice, two loci were identified with significant linkage to peak bone mass, one on Chromosome (Chr) 11 and another on Chr 13, with a maximum lod score of 10.8 (22.2% of the total variance) and 5.8 (10.0%), respectively. Another locus on the X Chr was suggestive of a QTL associated oppositely with a low peak bone mass to the SAMP2 allele. This association was consistent with the distribution of peak bone mass in the F1 and F2. These findings should be useful to elucidate the genetics of osteoporosis.

Copyright information

© Springer-Verlag New York Inc. 1999

Authors and Affiliations

  • Motoyuki  Shimizu
    • 1
  • Keiichi  Higuchi
    • 2
  • Beth  Bennett
    • 3
  • Chen  Xia
    • 4
  • Tadao  Tsuboyama
    • 1
  • Soichiro  Kasai
    • 1
  • Takuya  Chiba
    • 4
  • Hiromi  Fujisawa
    • 4
  • Kumiko  Kogishi
    • 4
  • Haruo  Kitado
    • 5
  • Mitsutoshi  Kimoto
    • 1
  • Norikazu  Takeda
    • 1
  • Mutsumi  Matsushita
    • 1
  • Hideo  Okumura
    • 6
  • Tadao  Serikawa
    • 7
  • Takashi  Nakamura
    • 1
  • Thomas E.  Johnson
    • 3
  • Masanori  Hosokawa
    • 4
  1. 1.Department of Orthopedic Surgery, Faculty of Medicine, Kyoto University, Sakyo-ku, Kyoto 606-8507, JapanJP
  2. 2.Department of Aging Angiology, Research Center for Aging and Adaptation, Shinshu University School of Medicine, Matsumoto 390-8621, JapanJP
  3. 3.Institute for Behavioral Genetics, University of Colorado, Boulder 80309-0447, USAUS
  4. 4.Department of Senescence Biology, Chest Disease Research Institute, Kyoto University, Sakyo-ku, Kyoto 606-8507, JapanJP
  5. 5.Lilly Research Laboratories, Eli Lilly Japan K.K., Kobe 651-0086, JapanJP
  6. 6.Department of Orthopedic Surgery, Ehime University School of Medicine, Matsuyama 791-0295, JapanJP
  7. 7.Institute of Laboratory Animals, Faculty of Medicine, Kyoto University, Sakyo-ku, Kyoto 606-8501, JapanJP