Mammalian Genome

, Volume 10, Issue 1, pp 54–56

Genomic organization and chromosomal localization of the Itm2a gene

  • K.  Pittois
  • J.  Wauters
  • P.  Bossuyt
  • W.  Deleersnijder
  • J.  Merregaert

DOI: 10.1007/s003359900942

Cite this article as:
Pittois, K., Wauters, J., Bossuyt, P. et al. (1999) 10: 54. doi:10.1007/s003359900942

Abstract.

Itm2A is a novel type II integral membrane protein that is involved in osteo- and chondrogenic differentiation. Itm2a cDNA was originally isolated from a cDNA library of organ cultures from prenatal mouse mandibular condyles, by subtractive hybridization and differential screening. The Itm2a gene was isolated from a BALB/c liver genomic library. In total, 9.4 kb of the gene were sequenced, of which 2649 bp are 5′ flanking sequences. The Itm2a gene contains six exons and five introns. The splice sites conform to the GT/AG rule. The 5′ flanking region, which contains the presumed promoter sequence, lacks the common TATAA and CCAAT sequences, but contains consensus binding sites for various transcription factors. Several of these transcription factors are known to play a role in transcriptional regulation of cartilage- or bone-specific genes (e.g. Cbfa1, Cart-1, MHox, HES-1, and CIIS1). Itm2a was mapped to mouse chromosome position XA2-XA3 by fluorescent in situ hybridization (FISH) analysis. The human homolog, ITM2A, was mapped to chromosome position Xq13.3-Xq21.2.

Copyright information

© Springer-Verlag New York Inc. 1999

Authors and Affiliations

  • K.  Pittois
    • 1
  • J.  Wauters
    • 2
  • P.  Bossuyt
    • 2
  • W.  Deleersnijder
    • 3
  • J.  Merregaert
    • 1
  1. 1.Laboratory of Molecular Biotechnology, Department of Biochemistry, University of Antwerp, Universiteitsplein 1, B-2610 Wilrijk, BelgiumBE
  2. 2.Laboratory of Cytogenetics, Department of Medical Genetics, University of Antwerp, Universiteitsplein 1, B-2610 Wilrijk, BelgiumBE
  3. 3.N.V. Innogenetics, Industriepark Zwijnaarde 7 box 4, B-9052 Gent, BelgiumBE