Mammalian Genome

, Volume 7, Issue 7, pp 490–496

Molecular analysis of a recombinational hotspot adjacent to Lmp2 gene in the mouse MHC: Fine location and chromatin structure

  • K. Mizuno
  • T. Koide
  • T. Sagai
  • K. Moriwaki
  • T. Shiroishi
Original Contribution

DOI: 10.1007/s003359900149

Cite this article as:
Mizuno, K., Koide, T., Sagai, T. et al. Mammalian Genome (1996) 7: 490. doi:10.1007/s003359900149
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Abstract

Meiotic recombinations in the proximal region of the mouse major histocompatibility complex (MHC) are clustered within certain segments of chromosome, known as hotspots. In this study, we found that one of such hotspots, previously mapped between the Pb and Ob genes, is located very close to the 3′ end of the Lmp2 gene, which encodes a subunit of a proteolytic proteasome. To analyze the molecular basis of the site specificity of hotspots, we examined the structure of the chromatin around this Lmp2 hotspot and another one located in the MHC class II Eb gene, by monitoring DNase I-hypersensitive sites (DHSSs) of the chromatin. DHSSs were detected at the both hotspots in the somatic cells. In the meiotic cells, DHSS was detected within the Eb hotspot, as previously reported, but not in the Lmp2 hotspot. Thus, open structure of chromatin during meiosis, as monitored by hypersensitivity to DNase I, is not a general feature of mouse recombinational hotspots, contrasting the case of the lower eukaryote, S. cerevisiae, in which hotspots are always associated with DHSSs.

Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • K. Mizuno
    • 1
    • 2
    • 3
  • T. Koide
    • 2
  • T. Sagai
    • 2
  • K. Moriwaki
    • 1
  • T. Shiroishi
    • 2
  1. 1.Department of Genetics, School of Life ScienceThe Graduate University for Advanced StudiesShizuokaJapan
  2. 2.Mammalian Genetics LaboratoryNational Institute of GeneticsShizuokaJapan
  3. 3.Laboratory of Cellular and Molecular BiologyThe Institute of Physical and Chemical Research (RIKEN)Wako, SaitamaJapan

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