Mammalian Genome

, Volume 13, Issue 5, pp 234–238

Influences of inbreeding and genetics on telomere length in mice

  • Erin L. Manning
  • Janet Crossland
  • Michael J. Dewey
  • Gary Van Zant
Article

DOI: 10.1007/s003350020027

Cite this article as:
Manning, E., Crossland, J., Dewey, M. et al. Incorporating Mouse Genome (2002) 13: 234. doi:10.1007/s003350020027

Abstract.

We measured telomere lengths of blood leukocytes in several inbred and outbred mammalian species, using a telomere-specific fluorescent probe and flow cytometry. Humans, non-human primates, and three outbred populations of Peromyscus mice (Peromyscus leucopus, Peromyscus maniculatus, and Peromyscus polionotus) have short telomeres. Two common strains of laboratory mice, C57BL/6J and DBA/2J, have telomeres several times longer than most other mammals surveyed. Moreover, the two inbred laboratory mouse strains display significantly different telomere lengths, suggesting the existence of strain-specific genetic determinants. To further examine the effects of inbreeding, we studied three Peromyscus leucopus inbred lines (GS109, GS16A1, and GS16B), all derived from the outbred P. leucopus stock. Telomeres of all three inbred lines are significantly lengthened relative to outbred P. leucopus, and the three lines display strain-specific significantly different telomere lengths, much like the C57BL/6J and DBA/2J strains of M. musculus. To further characterize the genetic inheritance of telomere length, we carried out several crosses to obtain hybrid F1 mice between parental strains displaying the phenotype of long and short telomeres. In all F1 mice assayed, peripheral blood leukocyte telomere length was intermediate to that of the parents. Additionally, we generated F2 mice from a cross of the (P. leucopus outbred × GS16B)F1. Based on the distribution of telomere length in the F2 population, we determined that more than five loci contribute to telomere length regulation in Peromyscus. We concluded that inbreeding, through unknown mechanisms, results in the elongation of telomeres, and that telomere length for a given species and/or sub-strain is genetically determined by multiple segregating loci.

Copyright information

© Springer-Verlag New York Inc. 2002

Authors and Affiliations

  • Erin L. Manning
    • 1
  • Janet Crossland
    • 2
  • Michael J. Dewey
    • 2
  • Gary Van Zant
    • 1
  1. 1.Departments of Physiology and Internal Medicine, Division of Hematology/Oncology, Markey Cancer Center, Room CC-408, University of Kentucky, 800 Rose St., Lexington, Kentucky 40536-0093, USAUS
  2. 2.Department of Biological Sciences and Peromyscus Stock Center, University of South Carolina, Columbia, South Carolina 29208, USAUS