Mammalian Genome

, Volume 12, Issue 4, pp 309–317

Comparative analysis of human, bovine, and murine Oct-4 upstream promoter sequences

  • Verena  Nordhoff
  • Karin  Hübner
  • Andrea  Bauer
  • Irina  Orlova
  • Areti  Malapetsa
  • Hans R.  Schöler
Article

DOI: 10.1007/s003350010279

Cite this article as:
Nordhoff, V., Hübner, K., Bauer, A. et al. Incorporating Mouse Genome (2001) 12: 309. doi:10.1007/s003350010279

Abstract.

The Oct-4 gene encodes a transcription factor that is specifically expressed in embryonic stem cells and germ cells of the mouse embryo. Cells that differentiate into somatic tissues lose Oct-4 expression. Regulation of Oct-4 gene transcription involves a TATA-less minimal promoter and two upstream elements: the proximal (PE) and distal enhancers (DE). We report here the nucleotide sequence of the 5′ upstream regulatory regions of the human and murine Oct-4 genes. A comparative alignment analysis between these regions and those of the bovine Oct-4 ortholog reveals four conserved regions of homology (CR 1 to 4) between these species (66–94% conservation). The 1A sequence within the mouse PE is located approximately half-way between CR 2 and CR 3. A putative Sp1/Sp3 binding site and the overlapping hormone responsive element (HRE) in CR 1 are identical in all three species. A high number of CCC(A/T)CCC motifs exhibit various levels of homology in these upstream regions. We discuss the importance of these and other sequences and present candidate factors that may bind and regulate Oct-4 gene expression.

Copyright information

© Springer-Verlag New York Inc. 2001

Authors and Affiliations

  • Verena  Nordhoff
    • 1
  • Karin  Hübner
    • 1
  • Andrea  Bauer
    • 2
  • Irina  Orlova
    • 1
  • Areti  Malapetsa
    • 1
  • Hans R.  Schöler
    • 1
  1. 1.The Center for Animal Transgenesis and Germ Cell Research, New Bolton Center, School of Veterinary Medicine, University of Pennsylvania, 382 West Street Road, Kennett Square, Pennsylvania 19348, USAUS
  2. 2.EMBL, Gene Expression Programme, Meyerhofstrasse 1, D-69126, Heidelberg, GermanyDE