Mammalian Genome

, Volume 12, Issue 1, pp 13–16

High-resolution genetic mapping of the saccharin preference locus (Sac) and the putative sweet taste receptor (T1R1) gene (Gpr70) to mouse distal Chromosome 4

  • Xia  Li
  • Masashi  Inoue
  • Danielle R.  Reed
  • Taufiqul  Huque
  • Ralph B.  Puchalski
  • Michael G.  Tordoff
  • Yuzo  Ninomiya
  • Gary K.  Beauchamp
  • Alexander A.  Bachmanov

DOI: 10.1007/s003350010236

Cite this article as:
Li, X., Inoue, M., Reed, D. et al. (2001) 12: 13. doi:10.1007/s003350010236
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Abstract.

The Sac (saccharin preference) locus affecting mouse behavioral and neural responsiveness to sweeteners has been mapped to distal Chr 4. A putative sweet taste receptor, T1R1, has been recently cloned, and the gene encoding it, Gpr70, has also been mapped to mouse distal Chr 4. To assess Gpr70 as a candidate gene for Sac, we compared the Gpr70 sequences of C57BL/6ByJ and 129P3/J mouse strains with different alleles of Sac. Using Gpr70 sequence variation between the C57BL/6ByJ and 129P3/J strains, we conducted a high-resolution analysis of the chromosomal localization of the Gpr70 and Sac loci in the F2 hybrids and 129.B6-Sac partially congenic mice originating from these two strains. The Gpr70 gene maps proximal to Sac, which demonstrates that they are different loci.

Copyright information

© Springer-Verlag New York Inc. 2001

Authors and Affiliations

  • Xia  Li
    • 1
  • Masashi  Inoue
    • 2
  • Danielle R.  Reed
    • 1
  • Taufiqul  Huque
    • 1
  • Ralph B.  Puchalski
    • 1
  • Michael G.  Tordoff
    • 1
  • Yuzo  Ninomiya
    • 3
  • Gary K.  Beauchamp
    • 1
  • Alexander A.  Bachmanov
    • 1
  1. 1.Monell Chemical Senses Center, 3500 Market Street, Philadelphia, Pennsylvania 19104, USAUS
  2. 2.Laboratory of Cellular Neurobiology, School of Life Science, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo 192-03, JapanJP
  3. 3.Department of Oral Physiology, Faculty of Dentistry, Kyushu University, 3-1-1, Maidashi, Higashi-Ku, Fukuoka 812-8582, JapanJP
  4. 4.Department of Psychology and School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USAUS

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