Mammalian Genome

, Volume 11, Issue 11, pp 972–978

The hormone-sensitive lipase gene is transcribed from at least five alternative first exons in mouse adipose tissue

Authors

  • Nancy N.  Laurin
    • Medical Genetics Service, Research Center, Sainte-Justine Hospital, 3175 Côte Ste-Catherine, Montreal (Quebec) H3T 1C5, Canada
  • Shu Pei  Wang
    • Medical Genetics Service, Research Center, Sainte-Justine Hospital, 3175 Côte Ste-Catherine, Montreal (Quebec) H3T 1C5, Canada
  • Grant A.  Mitchell
    • Medical Genetics Service, Research Center, Sainte-Justine Hospital, 3175 Côte Ste-Catherine, Montreal (Quebec) H3T 1C5, Canada

DOI: 10.1007/s003350010185

Cite this article as:
Laurin, N., Wang, S. & Mitchell, G. (2000) 11: 972. doi:10.1007/s003350010185

Abstract.

Hormone-sensitive lipase (HSL) mediates triglyceride hydrolysis in adipocytes, in which its expression varies with physiological stress and is controlled posttranslationally and transcriptionally. We sequenced the mouse HSL gene for 8.2 kb upstream of the translation start codon and studied the steady-state HSL mRNA levels in mouse adipose tissue. In 50 clones derived from primer extension and PCR of mouse adipose cDNA, we found five distinct 5′ extremities that correspond to distinct exons in genomic DNA. Exon A is located ∼7 kb 5′ to the HSL translation start site. Exons B, C, and D are clustered 1.5–2 kb upstream, and the previously described exon 1 is immediately upstream and contiguous with the previously described HSL translation start site. Exon A is located ∼7 kb upstream and contains an in-frame methionine codon that could potentially generate another HSL isoform with 43 additional N-terminal residues. cDNA clones containing the newly described exons suggested that each exon has several transcription start sites but that all splice to an acceptor site located 20 nt upstream of the translation initiation codon in exon 1. HSL transcription in mouse adipose tissue originates from multiple sites in the 7-kb region between exon A and exon 1, with peaks at exon C (50–70% of HSL transcripts), exon 1 (5–30%), and exon A (∼10%). There are multiple potential transcription factor-binding elements upstream of each exon, suggesting the possibility of differential transcriptional regulation of HSL in different tissues and under various physiologic conditions.

Copyright information

© Springer-Verlag New York Inc. 2000