Mammalian Genome

, Volume 11, Issue 1, pp 2–7

Mapping of obesity QTLs in a cross between mouse lines divergently selected on fat content

Authors

  • Simon  Horvat
    • Roslin Institute (Edinburgh), Division of Molecular Biology, Roslin EH25 9PS, Scotland, UK
  • Lutz  Bünger
    • Institute of Cell, Animal and Population Biology, University of Edinburgh, West Mains Road, Edinburgh, EH9 3JT, Scotland, UK
  • Victoria M.  Falconer
    • Roslin Institute (Edinburgh), Division of Molecular Biology, Roslin EH25 9PS, Scotland, UK
  • Pamela  Mackay
    • Roslin Institute (Edinburgh), Division of Molecular Biology, Roslin EH25 9PS, Scotland, UK
  • Andy  Law
    • Roslin Institute (Edinburgh), Division of Molecular Biology, Roslin EH25 9PS, Scotland, UK
  • Grahame  Bulfield
    • Roslin Institute (Edinburgh), Division of Molecular Biology, Roslin EH25 9PS, Scotland, UK
  • Peter D.  Keightley
    • Institute of Cell, Animal and Population Biology, University of Edinburgh, West Mains Road, Edinburgh, EH9 3JT, Scotland, UK
Article

DOI: 10.1007/s003350010002

Cite this article as:
Horvat, S., Bünger, L., Falconer, V. et al. Incorporating Mouse Genome (2000) 11: 2. doi:10.1007/s003350010002

Abstract.

A genome-wide quantitative trait locus (QTL) analysis was performed in a polygenic obesity mouse model resulting from a long-term selection experiment. The parental lines were outbred lines divergently selected for 53 generations for high-fat (fat, F line) or low-fat (lean, L line) percentage (fat%) that differed fivefold in fat% at 14 weeks of age. An F2 population of 436 mice was used for the QTL analysis with 71 markers distributed across the genome. The analysis revealed significant QTLs Fob1 (for F-line obesity QTL 1), Fob2, Fob3, and Fob4, on Chromosomes (Chrs) 2, 12, 15, and X, respectively. None of these QTLs map to regions of known single gene obesity mutations (Lepob, Leprdb, Cpefat, Ay, tub), though they map to regions of previously described obesity QTLs and candidate genes. The effects of Fob1, Fob3, Fob4 were additive, and that of Fob2 was dominant. Fob2 also showed a significant female-specific effect. Fob1, Fob2, Fob3, and Fob4 explained 4.9%, 19.5%, 14.4%, and 7.3% of the F2 phenotypic variance for fat%, respectively. This study identified four loci that contributed to the response to divergent selection and control a significant proportion of the difference in obesity between the F and L lines.

Copyright information

© Springer-Verlag New York Inc. 2000