Article

Mammalian Genome

, 20:720

First online:

Genetic loci that regulate healing and regeneration in LG/J and SM/J mice

  • Elizabeth P. BlankenhornAffiliated withDepartment of Microbiology and Immunology, Drexel University College of Medicine Email author 
  • , Gregory BryanAffiliated withDepartment of Microbiology and Immunology, Drexel University College of Medicine
  • , Andrew V. KossenkovAffiliated withThe Wistar Institute
  • , Lise Desquenne ClarkAffiliated withThe Wistar Institute
  • , Xiang-Ming ZhangAffiliated withThe Wistar Institute
  • , Celia ChangAffiliated withThe Wistar Institute
  • , Wenhwai HorngAffiliated withThe Wistar Institute
  • , L. Susan PletscherAffiliated withDepartment of Anatomy and Neurobiology, Washington University School of Medicine
  • , James M. CheverudAffiliated withDepartment of Anatomy and Neurobiology, Washington University School of Medicine
    • , Louise C. ShoweAffiliated withThe Wistar Institute
    • , Ellen Heber-KatzAffiliated withThe Wistar Institute Email author 

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Abstract

MRL mice display unusual healing properties. When MRL ear pinnae are hole punched, the holes close completely without scarring, with regrowth of cartilage and reappearance of both hair follicles and sebaceous glands. Studies using (MRL/lpr × C57BL/6)F2 and backcross mice first showed that this phenomenon was genetically determined and that multiple loci contributed to this quantitative trait. The lpr mutation itself, however, was not one of them. In the present study we examined the genetic basis of healing in the Large (LG/J) mouse strain, a parent of the MRL mouse and a strain that shows the same healing phenotype. LG/J mice were crossed with Small (SM/J) mice and the F2 population was scored for healing and their genotypes determined at more than 200 polymorphic markers. As we previously observed for MRL and (MRL × B6)F2 mice, the wound-healing phenotype was sexually dimorphic, with female mice healing more quickly and more completely than male mice. We found quantitative trait loci (QTLs) on chromosomes (Chrs) 9, 10, 11, and 15. The heal QTLs on Chrs 11 and 15 were linked to differential healing primarily in male animals, whereas QTLs on Chrs 9 and 10 were not sexually dimorphic. A comparison of loci identified in previous crosses with those in the present report using LG/J × SM/J showed that loci on Chrs 9, 11, and 15 colocalized with those seen in previous MRL crosses, whereas the locus on Chr 10 was not seen before and is contributed by SM/J.