Mammalian Genome

, Volume 20, Issue 1, pp 14–20

Establishment of germline-competent embryonic stem cell lines from the MSM/Ms strain

Authors

  • Kimi Araki
    • Department of Developmental Genetics, Institute of Molecular Embryology and GeneticsKumamoto University
  • Naoki Takeda
    • Institute of Resource Development and AnalysisKumamoto University
  • Atsushi Yoshiki
    • Riken Bioresource Center
  • Yuichi Obata
    • Riken Bioresource Center
  • Naomi Nakagata
    • Institute of Resource Development and AnalysisKumamoto University
  • Toshihiko Shiroishi
    • National Institute of Genetics
  • Kazuo Moriwaki
    • Riken Bioresource Center
    • Department of Developmental Genetics, Institute of Molecular Embryology and GeneticsKumamoto University
Article

DOI: 10.1007/s00335-008-9160-7

Cite this article as:
Araki, K., Takeda, N., Yoshiki, A. et al. Mamm Genome (2009) 20: 14. doi:10.1007/s00335-008-9160-7

Abstract

MSM/Ms is an inbred mouse strain established from the Japanese wild mouse, Mus musculus molossinus, which has been phylogenetically distinct from common laboratory mouse strains for about 1 million years. The nucleotide substitution rate between MSM/Ms and C57BL/6 is estimated to be 0.96%. MSM/Ms mice display unique characteristics not observed in the commonly used laboratory strains, including an extremely low incidence of tumor development, high locomotor activity, and resistance to high-fat-diet-induced diabetes. Thus, functional genomic analyses using MSM/Ms should provide a powerful tool for the identification of novel phenotypes and gene functions. We report here the derivation of germline-competent embryonic stem (ES) cell lines from MSM/Ms blastocysts, allowing genetic manipulation of the M. m. molossinus genome. Fifteen blastocysts were cultured in ES cell medium and three ES lines, Mol/MSM-1, -2, and -3, were established. They were tested for germline competency by aggregation with ICR morulae and germline chimeras were obtained from all three lines. We also injected Mol/MSM-1 ES cells into blastocysts of ICR or C57BL/6 × BDF1 mice and found that blastocyst injection resulted in a higher production rate of chimeric mice than did aggregation. Furthermore, Mol/MSM-1 subclones electroporated with a gene trap vector were also highly efficient at producing germline chimeras using C57BL/6 × BDF1 blastocyst injection. This Mol/MSM-1 ES line should provide an excellent new tool allowing the genetic manipulation of the MSM/Ms genome.

Copyright information

© Springer Science+Business Media, LLC 2008