Mammalian Genome

, Volume 19, Issue 7, pp 581-586

First online:

piRNA-like RNAs in the marsupial Monodelphis domestica identify transcription clusters and likely marsupial transposon targets

  • Eric J. DevorAffiliated withMolecular Genetics and Biophysics, Integrated DNA Technologies Email author 
  • , Lingyan HuangAffiliated withMolecular Genetics and Biophysics, Integrated DNA Technologies
  • , Paul B. SamollowAffiliated withDepartment of Veterinary Integrative Biosciences, Texas A&M University

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PIWI-interacting RNAs (piRNAs) are a recently discovered class of small noncoding RNAs that have been detected in human, mouse, rat, zebrafish, and Drosophila genomes. We have utilized a size-directed small-RNA cloning procedure to clone and map more than 300 candidate piRNA-like small RNAs in the genome of the marsupial species Monodelphis domestica. Our results are consistent with those from other species in that the piRNA-like candidate sequences range in size from 28 to 31 nucleotides, show a pronounced preference for uridine at the 5′ end, are transcribed from a few large clusters, appear to target transposons, and display virtually no sequence conservation.