Mammalian Genome

, Volume 19, Issue 1, pp 26–31

Characterization of the ETnII-α endogenous retroviral element in the BALB/cJ Zhx2Afr1 allele

Authors

  • Sudhir Perincheri
    • Department of MicrobiologyImmunology and Molecular Genetics, University of Kentucky College of Medicine
    • Department of Genetics and DevelopmentColumbia University
  • David K. Peyton
    • Department of Biological and Environmental SciencesMorehead State University
  • Michelle Glenn
    • Department of MicrobiologyImmunology and Molecular Genetics, University of Kentucky College of Medicine
  • Martha L. Peterson
    • Department of MicrobiologyImmunology and Molecular Genetics, University of Kentucky College of Medicine
    • Markey Cancer CenterUniversity of Kentucky College of Medicine
    • Department of MicrobiologyImmunology and Molecular Genetics, University of Kentucky College of Medicine
    • Markey Cancer CenterUniversity of Kentucky College of Medicine
    • Department of Microbiology, Immunology and Molecular Genetics, 210 Combs BuildingMarkey Cancer Center, University of Kentucky College of Medicine
Article

DOI: 10.1007/s00335-007-9077-6

Cite this article as:
Perincheri, S., Peyton, D.K., Glenn, M. et al. Mamm Genome (2008) 19: 26. doi:10.1007/s00335-007-9077-6

Abstract

Integration of mouse endogenous retroviral (MERV) elements is responsible for an estimated 10% of spontaneous mutations that have been characterized in the laboratory mouse. We recently identified a MERV integration in the first intron of the Zinc fingers and homeoboxes 2 (Zhx2) gene in BALB/cJ mice, resulting in reduced Zhx2 expression. This integration is found in BALB/cJ but not in other BALB/c substrains, indicating that it occurred after these substrains separated in the late 1930s. We have characterized this MERV element and show here that it belongs to the ETnII-α class of elements. Our analysis reveals that the Zhx2 ETn element lacks a 69-bp sequence compared to most other ETn elements which may be due to recombination between two identical 13-bp elements. Three mature Zhx2 transcripts are found in the liver of BALB/cJ mice. The major transcript is spliced from Zhx2 exon 1 to the 5′ ETn LTR and is polyadenylated at the 3′ LTR. Of the two less abundant transcripts, one is identical to the wild-type transcript, whereas the second contains 183 bp of ETn sequence spliced between Zhx2 exons 1 and 2. We have also sequenced and analyzed products from the faslpr ETn found in MRL/lpr mice and show that it belongs to the ETnII-β class of elements.

Copyright information

© Springer Science+Business Media, LLC 2007