Mammalian Genome

, Volume 16, Issue 11, pp 829–837

Implementation of the modified-SHIRPA protocol for screening of dominant phenotypes in a large-scale ENU mutagenesis program

Authors

  • Hiroshi Masuya
    • Functional Genomics Research GroupRIKEN Genomic Sciences Center
  • Maki Inoue
    • Functional Genomics Research GroupRIKEN Genomic Sciences Center
  • Yumiko Wada
    • Functional Genomics Research GroupRIKEN Genomic Sciences Center
  • Aya Shimizu
    • Functional Genomics Research GroupRIKEN Genomic Sciences Center
  • Junko Nagano
    • Functional Genomics Research GroupRIKEN Genomic Sciences Center
  • Akiko Kawai
    • Functional Genomics Research GroupRIKEN Genomic Sciences Center
  • Ayako Inoue
    • Functional Genomics Research GroupRIKEN Genomic Sciences Center
  • Tomoko Kagami
    • Functional Genomics Research GroupRIKEN Genomic Sciences Center
  • Taeko Hirayama
    • Functional Genomics Research GroupRIKEN Genomic Sciences Center
  • Ayako Yamaga
    • Functional Genomics Research GroupRIKEN Genomic Sciences Center
  • Hideki Kaneda
    • Functional Genomics Research GroupRIKEN Genomic Sciences Center
  • Kimio Kobayashi
    • Functional Genomics Research GroupRIKEN Genomic Sciences Center
  • Osamu Minowa
    • Functional Genomics Research GroupRIKEN Genomic Sciences Center
  • Ikuo Miura
    • Functional Genomics Research GroupRIKEN Genomic Sciences Center
  • Yoichi Gondo
    • Population and Quantitative Genomics TeamRIKEN Genomic Sciences Center
  • Tetsuo Noda
    • Functional Genomics Research GroupRIKEN Genomic Sciences Center
  • Shigeharu Wakana
    • Functional Genomics Research GroupRIKEN Genomic Sciences Center
    • Functional Genomics Research GroupRIKEN Genomic Sciences Center
Original Contributions

DOI: 10.1007/s00335-005-2430-8

Cite this article as:
Masuya, H., Inoue, M., Wada, Y. et al. Mamm Genome (2005) 16: 829. doi:10.1007/s00335-005-2430-8

Abstract

SHIRPA is a three-stage protocol for the comprehensive assessment of primarily mouse behavior. The first stage consists of high-throughput phenotyping of 33 behavioral observations and 7 metabolic or disease observations. We modified this part of the protocol by integrating new morphologic observations into the initial phenotype assay of behavior and dysmorphology. Behavioral observations assessed by this protocol, now referred to as the “modified-SHIRPA,” are compatible with the original “SHIRPA” protocol. Using modified-SHIRPA, we screened dominant phenotypes of more than 10,000 G1 progeny generated by crossing DBA/2J females with ENU-treated C57BL/6J males. To date, we have obtained 136 hereditary-confirmed mutants that exhibit behavioral and morphologic defects. Some independent mutant lines exhibited similar phenotypes, suggesting that they may represent alleles of the same gene or mutations in the same genetic pathway. They could hold great potential for the unraveling of the molecular mechanisms of certain phenotypes.

Copyright information

© Springer Science+Business Media, Inc. 2005