Mammalian Genome

, Volume 16, Issue 11, pp 829–837

Implementation of the modified-SHIRPA protocol for screening of dominant phenotypes in a large-scale ENU mutagenesis program

  • Hiroshi Masuya
  • Maki Inoue
  • Yumiko Wada
  • Aya Shimizu
  • Junko Nagano
  • Akiko Kawai
  • Ayako Inoue
  • Tomoko Kagami
  • Taeko Hirayama
  • Ayako Yamaga
  • Hideki Kaneda
  • Kimio Kobayashi
  • Osamu Minowa
  • Ikuo Miura
  • Yoichi Gondo
  • Tetsuo Noda
  • Shigeharu Wakana
  • Toshihiko Shiroishi
Original Contributions

DOI: 10.1007/s00335-005-2430-8

Cite this article as:
Masuya, H., Inoue, M., Wada, Y. et al. Mamm Genome (2005) 16: 829. doi:10.1007/s00335-005-2430-8

Abstract

SHIRPA is a three-stage protocol for the comprehensive assessment of primarily mouse behavior. The first stage consists of high-throughput phenotyping of 33 behavioral observations and 7 metabolic or disease observations. We modified this part of the protocol by integrating new morphologic observations into the initial phenotype assay of behavior and dysmorphology. Behavioral observations assessed by this protocol, now referred to as the “modified-SHIRPA,” are compatible with the original “SHIRPA” protocol. Using modified-SHIRPA, we screened dominant phenotypes of more than 10,000 G1 progeny generated by crossing DBA/2J females with ENU-treated C57BL/6J males. To date, we have obtained 136 hereditary-confirmed mutants that exhibit behavioral and morphologic defects. Some independent mutant lines exhibited similar phenotypes, suggesting that they may represent alleles of the same gene or mutations in the same genetic pathway. They could hold great potential for the unraveling of the molecular mechanisms of certain phenotypes.

Copyright information

© Springer Science+Business Media, Inc. 2005

Authors and Affiliations

  • Hiroshi Masuya
    • 1
  • Maki Inoue
    • 1
  • Yumiko Wada
    • 1
  • Aya Shimizu
    • 1
  • Junko Nagano
    • 1
  • Akiko Kawai
    • 1
  • Ayako Inoue
    • 1
  • Tomoko Kagami
    • 1
  • Taeko Hirayama
    • 1
  • Ayako Yamaga
    • 1
  • Hideki Kaneda
    • 1
  • Kimio Kobayashi
    • 1
  • Osamu Minowa
    • 1
  • Ikuo Miura
    • 1
  • Yoichi Gondo
    • 2
  • Tetsuo Noda
    • 1
  • Shigeharu Wakana
    • 1
  • Toshihiko Shiroishi
    • 1
  1. 1.Functional Genomics Research GroupRIKEN Genomic Sciences CenterTsukubaiJapan
  2. 2.Population and Quantitative Genomics TeamRIKEN Genomic Sciences CenterYokohama CityJapan