Mammalian Genome

, Volume 17, Issue 1, pp 58–66

The mutation causing the black-and-tan pigmentation phenotype of Mangalitza pigs maps to the porcine ASIP locus but does not affect its coding sequence

  • Cord Drögemüller
  • Alexander Giese
  • Flávia Martins-Wess
  • Sabine Wiedemann
  • Leif Andersson
  • Bertram Brenig
  • Ruedi Fries
  • Tosso Leeb
Original Contributions

DOI: 10.1007/s00335-005-0104-1

Cite this article as:
Drögemüller, C., Giese, A., Martins-Wess, F. et al. Mamm Genome (2006) 17: 58. doi:10.1007/s00335-005-0104-1

Abstract

The gene for agouti signaling protein (ASIP) is centrally involved in the expression of coat color traits in animals. The Mangalitza pig breed is characterized by a black-and-tan phenotype with black dorsal pigmentation and yellow or white ventral pigmentation. We investigated a Mangalitza × Piétrain cross and observed a coat color segregation pattern in the F2 generation that can be explained by virtue of two alleles at the MC1R locus and two alleles at the ASIP locus. Complete linkage of the black-and-tan phenotype to microsatellite alleles at the ASIP locus on SSC 17q21 was observed. Corroborated by the knowledge of similar mouse coat color mutants, it seems therefore conceivable that the black-and-tan pigmentation of Mangalitza pigs is caused by an ASIP allele at, which is recessive to the wild-type allele A. Toward positional cloning of the at mutation, a 200-kb genomic BAC/PAC contig of this chromosomal region has been constructed and subsequently sequenced. Full-length ASIP cDNAs obtained by RACE differed in their 5′ untranslated regions, whereas they shared a common open reading frame. Comparative sequencing of all ASIP exons and ASIP cDNAs between Mangalitza and Piétrain pigs did not reveal any differences associated with the coat color phenotype. Relative qRT-PCR analyses showed different dorsoventral skin expression intensities of the five ASIP transcripts in black-and-tan Mangalitza. The at mutation is therefore probably a regulatory ASIP mutation that alters its dorsoventral expression pattern.

Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • Cord Drögemüller
    • 1
    • 2
  • Alexander Giese
    • 1
  • Flávia Martins-Wess
    • 1
  • Sabine Wiedemann
    • 3
  • Leif Andersson
    • 4
  • Bertram Brenig
    • 5
  • Ruedi Fries
    • 3
  • Tosso Leeb
    • 1
    • 2
  1. 1.Institute of Animal Breeding and GeneticsUniversity of Veterinary Medicine HannoverGermany
  2. 2.Institute of Genetics, Vetsuisse FacultyUniversity of BerneSwitzerland
  3. 3.Chair of Animal Breeding and Molecular GeneticsTechnical University of MunichGermany
  4. 4.Swedish University of Agricultural SciencesUppsalaSweden
  5. 5.Institute of Veterinary MedicineUniversity of GöttingenGermany