Mammalian Genome

, Volume 14, Issue 4, pp 223–230

Fine scale mapping of a genetic locus for conditioned fear

  • Christopher J. Talbot
  • Richard A. Radcliffe
  • Jan Fullerton
  • Robert Hitzemann
  • Jeanne M. Wehner
  • Jonathan Flint

DOI: 10.1007/s00335-002-3059-5

Cite this article as:
Talbot, C., Radcliffe, R., Fullerton, J. et al. Mamm Genome (2003) 14: 223. doi:10.1007/s00335-002-3059-5

Abstract

Fear conditioning is one of a number of models for investigating the genetic basis of individual variation in emotion and learning. Genetic mapping using crosses between strains of laboratory mice has identified a locus on chromosome one that appears to influence not only variation in conditioned fear, but also in other validated tests of fear-related behaviour, (including the open-field and the elevated-plus maze), suggesting that the rodent locus may act in ways consistent with how a locus influencing susceptibility to anxiety in humans is believed to operate. Here we use high-resolution mapping in genetically heterogeneous mice to show that a quantitative trait locus influencing conditioned fear can be separated from loci influencing open-field activity. Mapping in two different heterogeneous stocks, the Boulder and Northport HS, gave similar map locations for open-field activity at two positions on the current mouse physical map, one at 162 Mb on chromosome one (negative log P-value 5.4) the other at 173 Mb (negative log P-value 4.8), while mapping of contextual conditioned fear in the Boulder HS identified a locus at 170 Mb (negative log P-value 5.4). Estimates of the 95% confidence intervals show that the locations do not overlap. The region containing a gene or genes that influence variation in conditioned fear is approximately 1 megabase in size and contains only one gene of known function, a pre-B cell leukaemia factor.

Copyright information

© Springer-Verlag New York Inc. 2003

Authors and Affiliations

  • Christopher J. Talbot
    • 1
  • Richard A. Radcliffe
    • 2
  • Jan Fullerton
    • 4
  • Robert Hitzemann
    • 5
  • Jeanne M. Wehner
    • 2
  • Jonathan Flint
    • 4
  1. 1.Department of Genetics, University of Leicester, University Road, Leicester LE1 7RH, UK
  2. 2.Institute for Behavioral Genetics, University of Colorado, Boulder, Colorado 80309, USA
  3. 3.School of Pharmacy, University of Colorado Health Sciences Center, Denver, Colorado 80262, USA
  4. 4.Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford OX3 7BN, UK
  5. 5.Department of Behavioral Neuroscience, Oregon Health Sciences University, Portland, Oregon 97201-3098, USA