Mammalian Genome

, Volume 14, Issue 1, pp 21–30

Characterization of a putative type IV aminophospholipid transporter P-type ATPase

  • Stéphane  Flamant
  • Pascale  Pescher
  • Brigitte  Lemercier
  • Mathieu  Clément-Ziza
  • François  Képès
  • Marc  Fellous
  • Geneviève  Milon
  • Gilles  Marchal
  • Claude  Besmond

DOI: 10.1007/s00335-002-3032-3

Cite this article as:
Flamant, S., Pescher, P., Lemercier, B. et al. Mamm Genome (2003) 14: 21. doi:10.1007/s00335-002-3032-3

The P-type ATPases comprise a well-studied family of proteins involved in the active transport of charged substrates across biological membranes. Starting from a mouse bone marrow-derived macrophage cDNA library and using a signal peptide trapping strategy, we identified a new P-type ATPase family member. We characterized the genomic structure of this gene, named Atp10d, as well as its human counterpart. The presence of P-type ATPase consensus motifs and phylogenetic analysis showed that this gene is a member of the type IV, putative amphipath transporters subfamily. We showed that this gene is expressed in kidney and placenta. We also found that the C57BL/6 strain carries a constitutive stop codon in the sequence of Atp10d exon 12, whereas 14 other inbred mouse strains show an uninterrupted reading frame at this location. This mutation in C57BL/6 should lead to a non-functional protein, suggesting that this gene may not be essential. We discuss the involvement of the Atp10d gene in the fat-prone phenotype of the C57BL/6 strain and its physical mapping within a QTL associated with HDL-cholesterol levels.

Copyright information

© Springer-Verlag New York Inc. 2003

Authors and Affiliations

  • Stéphane  Flamant
    • 1
  • Pascale  Pescher
    • 2
  • Brigitte  Lemercier
    • 3
  • Mathieu  Clément-Ziza
    • 1
  • François  Képès
    • 4
  • Marc  Fellous
    • 3
  • Geneviève  Milon
    • 5
  • Gilles  Marchal
    • 2
  • Claude  Besmond
    • 1
  1. 1.Hôpital Necker-Enfants Malades, INSERM U393, Tour Lavoisier, 2 ème étage, 149 rue de Sèvres, 75015 Paris, FranceFR
  2. 2.Laboratoire de Référence des Mycobactéries, Institut Pasteur, 25 rue du Dr. Roux, 75015 Paris, FranceFR
  3. 3.Laboratoire d'Immunogénétique Humaine, Institut Pasteur, 25 rue du Dr. Roux, 75015 Paris, FranceFR
  4. 4.ATelier de Génomique Cognitive, CNRS ESA 8071/Génopole®, 523 Terrasses de l'Agora, 91000 Evry, FranceFR
  5. 5.Unité d'Immunophysiologie et Parasitisme Intracellulaire, Institut Pasteur, 25 rue du Dr. Roux, 75015 Paris, FranceFR