Mammalian Genome

, Volume 13, Issue 10, pp 569–577

A large duplication associated with dominant white color in pigs originated by homologous recombination between LINE elements flanking KIT

  • Elisabetta  Giuffra
  • Anna  Törnsten
  • Stefan  Marklund
  • Erik  Bongcam-Rudloff
  • Patrick  Chardon
  • James M.H. Kijas
  • Susan I. Anderson
  • Alan L. Archibald
  • Leif  Andersson

DOI: 10.1007/s00335-002-2184-5

Cite this article as:
Giuffra, E., Törnsten, A., Marklund, S. et al. Mamm Genome (2002) 13: 569. doi:10.1007/s00335-002-2184-5

The Dominant White (I/KIT) locus is one of the major coat color loci in the pig. Previous studies showed that the Dominant White (I) and Patch (IP) alleles are both associated with a duplication including the entire KIT coding sequence. We have now constructed a BAC contig spanning the three closely linked tyrosine kinase receptor genes PDGFRA–KIT–KDR. The size of the duplication was estimated at about 450 kb and includes KIT, but not PDGFRA and KDR. Sequence analysis revealed that the duplication arose by unequal homologous recombination between two LINE elements flanking KIT. The same unique duplication breakpoint was identified in animals carrying the I and IP alleles across breeds, implying that Dominant White and Patch alleles are descendants of a single duplication event. An unexpected finding was that Piétrain pigs carry the KIT duplication, since this breed was previously assumed to be wild type at this locus. Comparative sequence analysis indicated that the distinct phenotypic effect of the duplication occurs because the duplicated copy lacks some regulatory elements located more than 150 kb upstream of KIT exon 1 and necessary for normal KIT expression.

Copyright information

© Springer-Verlag New York Inc. 2002

Authors and Affiliations

  • Elisabetta  Giuffra
    • 1
  • Anna  Törnsten
    • 1
  • Stefan  Marklund
    • 1
  • Erik  Bongcam-Rudloff
    • 1
  • Patrick  Chardon
    • 2
  • James M.H. Kijas
    • 1
  • Susan I. Anderson
    • 3
  • Alan L. Archibald
    • 3
  • Leif  Andersson
    • 1
  1. 1.Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Box 597, S-751 24 Uppsala, SwedenSE
  2. 2.Laboratoire de Radiobiologie et d'Etude du Génome, CEA INRA, F-78352 Jouy-en-Josas Cedex, FranceFR
  3. 3.Roslin Institute (Edinburgh), Roslin, Midlothian EH25 9PS, Scotland, UKGB