European Radiology

, Volume 22, Issue 8, pp 1641–1650

DNA double-strand breaks as potential indicators for the biological effects of ionising radiation exposure from cardiac CT and conventional coronary angiography: a randomised, controlled study

  • Dominik Geisel
  • Elke Zimmermann
  • Matthias Rief
  • Johannes Greupner
  • Michael Laule
  • Fabian Knebel
  • Bernd Hamm
  • Marc Dewey
Cardiac

DOI: 10.1007/s00330-012-2426-1

Cite this article as:
Geisel, D., Zimmermann, E., Rief, M. et al. Eur Radiol (2012) 22: 1641. doi:10.1007/s00330-012-2426-1

Abstract

Objectives

To prospectively compare induced DNA double-strand breaks by cardiac computed tomography (CT) and conventional coronary angiography (CCA).

Methods

56 patients with suspected coronary artery disease were randomised to undergo either CCA or cardiac CT. DNA double-strand breaks were assessed in fluorescence microscopy of blood lymphocytes as indicators of the biological effects of radiation exposure. Radiation doses were estimated using dose–length product (DLP) and dose–area product (DAP) with conversion factors for CT and CCA, respectively.

Results

On average there were 0.12 ± 0.06 induced double-strand breaks per lymphocyte for CT and 0.29 ± 0.18 for diagnostic CCA (P < 0.001). This relative biological effect of ionising radiation from CCA was 1.9 times higher (P < 0.001) than the effective dose estimated by conversion factors would have suggested. The correlation between the biological effects and the estimated radiation doses was excellent for CT (r = 0.951, P < 0.001) and moderate to good for CCA (r = 0.862, P < 0.001). One day after radiation, a complete repair of double-strand breaks to background levels was found in both groups.

Conclusions

Conversion factors may underestimate the relative biological effects of ionising radiation from CCA. DNA double-strand break assessment may provide a strategy for individualised assessments of radiation.

Key Points

• Radiation dose causes concern for both conventional coronary angiography and cardiac CT.

Estimations of the biological effects of ionising radiation may become feasible.

Fewer DNA double-strand breaks are induced by cardiac CT than CCA.

Conversion factors may underestimate the relative effects of ionising radiation from CCA.

Keywords

Heart catheterization X-ray computed tomography Double-stranded DNA breaks Radiation Relative biological effectiveness 

Abbreviations and Acronyms

CCA

conventional coronary angiography

DAP

dose–area product

DLP

dose–length product

DNA

deoxyribonucleic acid

DSB

DNA double-strand break

FCS

fetal calf serum

PBS

phosphate buffered saline

RPMI

Roswell Park Memorial Institute

Copyright information

© European Society of Radiology 2012

Authors and Affiliations

  • Dominik Geisel
    • 2
  • Elke Zimmermann
    • 2
  • Matthias Rief
    • 2
  • Johannes Greupner
    • 2
  • Michael Laule
    • 1
  • Fabian Knebel
    • 1
  • Bernd Hamm
    • 2
  • Marc Dewey
    • 2
    • 3
  1. 1.Department of CardiologyCharité Medical SchoolBerlinGermany
  2. 2.Department of RadiologyCharité Medical SchoolBerlinGermany
  3. 3.Charité, Institut für RadiologieBerlinGermany