European Radiology

, Volume 18, Issue 5, pp 993–1004

Comparison of continuous vs. pulsed focused ultrasound in treated muscle tissue as evaluated by magnetic resonance imaging, histological analysis, and microarray analysis

  • Walter Hundt
  • Esther L. Yuh
  • Silke Steinbach
  • Mark D. Bednarski
  • Samira Guccione
Molecular Imaging

DOI: 10.1007/s00330-007-0848-y

Cite this article as:
Hundt, W., Yuh, E.L., Steinbach, S. et al. Eur Radiol (2008) 18: 993. doi:10.1007/s00330-007-0848-y

Abstract

The purpose of this study was to investigate the effect of different application modes of high intensity focused ultrasound (HIFU) to muscle tissue. HIFU was applied to muscle tissue of the flank in C3H/Km mice. Two dose regimes were investigated, a continuous HIFU and a short-pulsed HIFU mode. Three hours after HIFU treatment pre- and post-contrast T1-weighted, T2-weighted images and a diffusion-weighted STEAM sequence were obtained. After MR imaging, the animals were euthenized and the treated, and the non-treated tissue was taken out for histology and functional genomic analysis. T2 images showed increased signal intensity and post-contrast T1 showed a decreased contrast uptake in the central parts throughout the tissue of both HIFU modes. A significantly higher diffusion coefficient was found in the muscle tissue treated with continuous wave focused ultrasound. Gene expression analysis revealed profound changes of 54 genes. For most of the analyzed genes higher expression was found after treatment with the short-pulse mode. The highest up-regulated genes encoded for the MHC class III (FC ≈84), HSP 70 (FC ≈75) and FBJ osteosarcoma related oncogene (FC ≈21). Immunohistology and the immunoblot analysis confirmed the presence of HSP70 protein in both applied HIFU modes. The use of HIFU treatment on muscle tissue results in dramatic changes in gene expression; however, the same genes are up-regulated after the application of continuous or pulsed HIFU, indicating that the tissue reaction is independent of the type of tissue damage.

Keywords

Focused ultrasoundMuscle tissueMRIHistologyGene expression

Copyright information

© European Society of Radiology 2008

Authors and Affiliations

  • Walter Hundt
    • 1
    • 2
  • Esther L. Yuh
    • 1
  • Silke Steinbach
    • 3
  • Mark D. Bednarski
    • 1
  • Samira Guccione
    • 1
  1. 1.Lucas MRS Research Center, Department of RadiologyStanford University School of MedicineStanfordUSA
  2. 2.Department of RadiologyLudwig Maximilians University of MunichMunichGermany
  3. 3.Department of Otolaryngology Head and Neck SurgeryTechnical University of MunichMunichGermany