Cold tolerance of Pseudomonas sp. 30-3 isolated from oil-contaminated soil, Antarctica
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Pseudomonas sp. 30-3 was enriched from oil-contaminated soil from Wright Valley, Antarctica using JP8 jet fuel as sole carbon source. This isolate exhibited tolerance to temperatures ranging from 0°C to 35°C when cultured in laboratory medium. In a freeze-thaw study, an 89% survival was observed when Pseudomonas sp. 30-3 was exposed to 4°C prior to freezing. PCR amplification of a 248-bp DNA fragment in Pseudomonas sp. 30-3 using capB-gene specific primers showed a 98% amino acid sequence homology with CapB of Pseudomonas fragi and 62% homology with CspA of Escherichia coli. Radiolabeling of total cellular proteins exhibited elevated expression of an 8-kDa protein at 4°C, which suggests that the CapB in Pseudomonas sp. 30-3 may play a pivotal role in survival and tolerance at cold and subzero temperatures. Tolerance to cold temperatures and the ability to degrade hydrocarbons by Pseudomonas sp. 30-3 provide support for the application of bioremediation for petroleum hydrocarbons in Antarctic soils.
- Cold tolerance of Pseudomonas sp. 30-3 isolated from oil-contaminated soil, Antarctica
Volume 25, Issue 1 , pp 5-11
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- A1. Department of Biology, University of Alabama at Birmingham, 1300 University Boulevard, Birmingham, AL 34294-1170, USA,
- A2. Landcare Research, Private Bag 3127, Hamilton, New Zealand,
- A3. School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland, New Zealand,