Polar Biology

, Volume 25, Issue 1, pp 5–11

Cold tolerance of Pseudomonas sp. 30-3 isolated from oil-contaminated soil, Antarctica

  • Gitika Panicker
  • Jackie Aislabie
  • David Saul
  • Asim K. Bej
Original Paper

DOI: 10.1007/s003000100304

Cite this article as:
Panicker, G., Aislabie, J., Saul, D. et al. Polar Biol (2002) 25: 5. doi:10.1007/s003000100304

Abstract.

Pseudomonas sp. 30-3 was enriched from oil-contaminated soil from Wright Valley, Antarctica using JP8 jet fuel as sole carbon source. This isolate exhibited tolerance to temperatures ranging from 0°C to 35°C when cultured in laboratory medium. In a freeze-thaw study, an 89% survival was observed when Pseudomonas sp. 30-3 was exposed to 4°C prior to freezing. PCR amplification of a 248-bp DNA fragment in Pseudomonas sp. 30-3 using capB-gene specific primers showed a 98% amino acid sequence homology with CapB of Pseudomonas fragi and 62% homology with CspA of Escherichia coli. Radiolabeling of total cellular proteins exhibited elevated expression of an 8-kDa protein at 4°C, which suggests that the CapB in Pseudomonas sp. 30-3 may play a pivotal role in survival and tolerance at cold and subzero temperatures. Tolerance to cold temperatures and the ability to degrade hydrocarbons by Pseudomonas sp. 30-3 provide support for the application of bioremediation for petroleum hydrocarbons in Antarctic soils.

Copyright information

© Springer-Verlag 2001

Authors and Affiliations

  • Gitika Panicker
    • 1
  • Jackie Aislabie
    • 2
  • David Saul
    • 3
  • Asim K. Bej
    • 1
  1. 1.Department of Biology, University of Alabama at Birmingham, 1300 University Boulevard, Birmingham, AL 34294-1170, USA
  2. 2.Landcare Research, Private Bag 3127, Hamilton, New Zealand
  3. 3.School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland, New Zealand