The role of summer precipitation and summer temperature in establishment and growth of dwarf shrub Betula nana in northeast Siberian tundra
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- Li, B., Heijmans, M.M.P.D., Berendse, F. et al. Polar Biol (2016) 39: 1245. doi:10.1007/s00300-015-1847-0
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It is widely believed that deciduous tundra-shrub dominance is increasing in the pan-Arctic region, mainly due to rising temperature. We sampled dwarf birch (Betula nana L.) at a northeastern Siberian tundra site and used dendrochronological methods to explore the relationship between climatic variables and local shrub dominance. We found that establishment of shrub ramets was positively related to summer precipitation, which implies that the current high dominance of B. nana at our study site could be related to high summer precipitation in the period from 1960 to 1990. The results confirmed that early summer temperature is most influential to annual growth rates of B. nana. In addition, summer precipitation stimulated shrub growth in years with warm summers, suggesting that B. nana growth may be co-limited by summer moisture supply. The dual controlling role of temperature and summer precipitation on B. nana growth and establishment is important to predict future climate-driven vegetation dynamics in the Arctic tundra.
KeywordsGlobal warming Arctic Shrub dominance Betula nana L. Dendrochronology Summer precipitation
Global climate change has markedly affected the ecosystems on Earth (ACIA 2005; IPCC 2013). Compared to other regions, the Arctic region is experiencing dramatic air temperature increase and more extreme precipitation events (Hinzman et al. 2005; McGuire et al. 2006). During the last four decades, surface air temperature increased on average 0.4 °C per decade over the Arctic (Anisimov et al. 2007).
As a consequence, local vegetation composition has changed in the North American and Scandinavian Arctic (ACIA 2005). In particular, deciduous shrubs have been observed to increase in Arctic tundra. Experimental studies in tundra ecosystems (Shaver et al. 2001; Mack et al. 2004; Walker et al. 2006; Bret-Harte et al. 2008; Nowinski et al. 2010) suggested that deciduous shrubs, especially Betula nana L. (dwarf birch), benefit from the increase in temperature, thereby becoming more dominant in the ecosystem. In the last 50 years, shrub-patch expansion was apparent at different sites throughout Alaska (Sturm et al. 2001; Tape et al. 2006), Canada (Hudson and Henry 2009) and other Arctic tundra regions (Myneni et al. 1997; Stow et al. 2004; Hudson and Henry 2009; Myers-Smith et al. 2011; Frost and Epstein 2014). The observed shrub expansion could reduce tundra albedo, leading to additional regional warming (Sturm et al. 2005; Blok et al. 2011b; Loranty et al. 2011; Juszak et al. 2014).
Field experiments, aerial photographs and remotely sensed data provided a general but coarse impression of tundra “greening” explained as shrub expansion during the last decades (Sturm et al. 2001). However, measurement errors caused by instrumentation drift, atmospheric effects (Stow et al. 2004) and low image resolution (Tape et al. 2006) limit the assessment of fine-scale shrub cover dynamics. Meanwhile, the time spans of most field warming experiments are too short to unravel the co-variation of shrub growth and local climate fluctuations (Bret-Harte et al. 2008).
Dendrochronology is a useful tool to explore the long-term relationship between climate and shrub growth and expansion (Bär et al. 2008; Myers-Smith et al. 2011, 2015b). Woody plants living under extreme conditions, such as shrubs in the Arctic, usually respond sensitively to climatic variability in their vertical and radial growth (Bradley and Jones 1992; Mäkinen et al. 2003; Myers-Smith et al. 2015a). Compared to trees, application of dendrochronology to Arctic deciduous shrubs is challenging mainly related to low growth rates and frequently missing rings (Woodcock and Bradley 1994) but also due to irregular wood formation along and around the main stem (Schweingruber et al. 2011, 2013).
Similar to other woody species in the Arctic tundra, B. nana forms exceptionally narrow annual tree rings (Groot et al. 1997; Meinardus et al. 2011; Hollesen et al. 2015). The average ring width of B. nana collected by Miller (1975) was merely 130 μm. Dendrochronological analyses of such material require application of serial sectioning, i.e., studying multiple samples along the main stem to account for frequently occurring missing rings, specifically in outer stem parts (Wilmking et al. 2012). Despite being a common circum-Arctic species that seems responsive to climate warming (Shaver et al. 2001; Wahren et al. 2005; Hollesen et al. 2015), B. nana has only recently been used for dendrochronological studies (Miller 1975; Blok et al. 2011a; Meinardus et al. 2011).
As temperature determines the length of the growing season in Arctic woody species (Walker et al. 2006; Hudson and Henry 2009; Berner et al. 2013), we assume that radial growth of B. nana at our site in the northeastern Siberian tundra is related to summer temperature. Pop et al. (2000) found that the bud break of B. nana is sensitive to spring air temperature and snowmelt. Since the growing season in the Arctic tundra is extremely short, an earlier start of the growing season usually results in increased growth. Precipitation may be crucial as well as neither waterlogged areas nor dry soil conditions favor B. nana growth (Groot et al. 1997). Precipitation could provide the main water supply for B. nana during the growing season, particularly when the shrubs grow on relatively well-drained soil or when the climate is dry. However, the growth of deciduous shrubs could also be restricted by extremely high summer precipitation due to anaerobic soil conditions that might develop (Lloyd et al. 2003). Aside from the rainfall in summer, winter precipitation (snow) can also affect shrub growth (Blok et al. 2015), as deeper snow cover in winter provides better insulation, which leads to higher turnover in soil organic matter, which is hypothesized to benefit shrub growth during the subsequent growing season (Sturm et al. 2001). The primary objectives of this study were to identify the main climatic factors that determine annual growth and establishment of B. nana, a common Arctic deciduous shrub species.
Material and methods
Site description and climate data
Long-term meteorological data (daily average temperature, daily cumulative precipitation and snow depth) since 1945 are available, recorded by the closest meteorological station (WMO station no. 21946 in Chokurdakh), 30 km south of the study site (http://climexp.knmi.nl/). The mean annual temperature (1981–2010) at Chokurdakh is −13.4 °C, and the mean annual precipitation is 196 mm (1981–2010) (Nauta et al. 2015). The mean July temperature of the same period is 10.3 °C. The mean temperature in January is −34.0 °C. Summer is defined as ranging from June to August, as the snow-free period usually lasts from the beginning of June until the end of August accordingly. For each year, the start of the growing season and length of the growing season were calculated using these Chokurdakh climate data.
Sample collection and preparation
In total, 90 B. nana ramets from 10 different B. nana-dominated patches (approximate 173 m2 in average) were systematically collected in July 2012. All 10 patches were located at a former thermokarst lake bed (Fig. 1), and the distance among patches was at least 30 m. In each patch, three transects (from center to patch edge) located at an angle of 120° were established. Along each transect, three ramet samples (center, middle and outside) were collected (online resource 1).
The sampling strategy in general followed the requirements for applying the serial-sectioning technique to ensure detection and measurements of all tree rings at the stem base and along the stem (Kolishchuk 1990). Individual ramet stems were cut just below the transition zone between root and stem (usually located a few centimeters below soil surface where the coarse roots started to appear; Fig. 1). After measuring the total length of a ramet, three 2- to 3-cm-thick disks were collected along the main growth axis: at the transition zone between root and stem (disk α), at the soil surface (disk β) and just below the first long branch (disk γ). All stem sections were stored immediately in glycerin–alcohol mixture (33 % glycerin, 33 % alcohol and 33 % water in volume). The samples were transported to Wageningen University for further processing.
In the Lab, a 15- to 20-µm-thick microsection was prepared from each disk of the three disks per ramet, using a GSL1 sledge microtome (Gärtner et al. 2014). For enhancing the contrast between different wood tissues which eases detection of ring boundaries, the sections were stained with a safranin/astra blue mixture. After 5-min staining, all the sections were dehydrated subsequently by using a 50, 96 and 99 % alcohol. Dehydrated samples were then washed with Roti®-Clear liquid and permanently imbedded in Roti®-Mount afterward. All the slides were eventually fastened on a steel plate by using two button-shaped magnets and air-dried for around 72 h.
Tree-ring measurement, cross-dating and chronology development
The mean ring-width series derived from the β disks were finally used to (1) determine when the individual ramets established and (2) to calculate a site chronology. For the latter, we used only individuals older than 30 years, as these are not only representing juvenile growth patterns, affected by initial competition-driven growth but mainly reflect environmental-driven growth patterns of grown up, established shrubs. Before a site chronology was calculated from these older ramets, the individual ring-width series were standardized in ARSTAN v6.05 to eliminate size- and age-related growth trends. After standardization, the index series are averaged into a site chronology (program ARSTAN) (Cook and Holmes 1986). The quality of this site chronology is checked by calculating the inter-series correlation (rbar) and the expressed signal (EPS) (Bunn 2008). Subsequently, the standardized ring-width index (RWI) was calculated. In addition, we compared the site chronology of B. nana in this study with the chronologies of B. nana and Salix pulchra Cham. in the study by Blok et al. (2011a). The RWI of B. nana (and S. pulchra) from both studies was compared as well.
Before analysis of climate–growth relationships, the normal distribution of each variable was checked by SPSS software (IBM SPSS Statistics for Windows, ver. 22.0; IBM Corp., Armonk, NY, USA). All variables showed a normal distribution, except some precipitation variables which were log-transformed to achieve or improve the normal distribution. Pearson’s correlation coefficients (r) were calculated for the relationships between the RWI chronology and different climatic factors: precipitation, temperature, snow depth, length of growing season (number of days with mean daily temperature over 5 °C per year) (Briffa et al. 2008) and the start date of the growing season. The start dates of the growing season during the period from 1952 to 2012 were calculated based on the equation developed by Pop et al. (2000). Parmentier et al. (2011) have calculated the start dates of the growing seasons of this region by using the same formula. In addition to precipitation, we also tested a drought index, which combines precipitation and temperature data. Standardized precipitation evapotranspiration index (SPEI, Vicente-Serrano et al. 2010) data were obtained for the study site location from the global SPEI database v2.3 (sac.csic.es/spei/database.html, Vicente-Serrano et al. 2010). Since climatic conditions can also affect next year’s radial growth (Buchwal et al. 2013), the Pearson’s correlations for the relation between RWI and the climatic variables of the preceding year were included into the analyses.
For each ramet, sprouting year was based on the number of measured rings on the β disks (from soil surface). From the sprouting years of all 90 samples, we obtained the annual establishment numbers. In order to explore the relationship between climate change and shrub ramet establishment, we used the generalized linear model that takes the Poisson distribution of count data into account (in SPSS) to examine the relation between the ramet establishment number (dependent variable) and climate variables (covariate). As the year-to-year variation, particularly in precipitation, is very large and the exact ages of the samples can be underestimated due to undetected missing rings, especially in very young individuals which are difficult to cross-date, we also used 5-year sums of establishment with 5-year averages of climate data to examine relationships between establishment and climate factors.
Age-related cambial growth and RWI chronology
Betula nana showed a pronounced negative logarithmic radial growth trend with increasing age (R2 = 0.85) (online resource 2). The ring-width increment declined sharply, especially during the first 15 years (slope = 9.68 μm year−1, R2 = 0.92), and this trend became much weaker in the later years (slope = −0.25 μm year−1, R2 = 0.19). The results from B. nana samples from Blok et al. (2011a) showed a similar result (online resource 2). In contrast to B. nana, the growth rate of S. pulchra showed a constant negative linear trend (slope = −2.68 μm year−1, R2 = 0.51) and ring widths were on average much larger (B. nana: 81.03 ± 38.91 μm, S. pulchra: 189.11 ± 61.59 μm) (online resource 2). Based on these 53 B. nana individuals, a final B. nana chronology was established. The chronology of B. nana was characterized by a reasonable common signal represented by rbar (0.351) and EPS (0.933). In addition, we observed that the ramet samples, which have been collected on July 20 in 2012, have final row(s) of flatted fibers (the ring boundaries of 2012).
Influence of climate factors on radial growth
Pearson’s correlation coefficient for relationships between annual ring-width index (RWI) of Betula nana and local climate variables (summer temperature (June–August), early summer temperature (June 16–July 20), precipitation (cumulative, June–August), mean snow depths (previous year’s October–May) and growing days and standardized precipitation evapotranspiration index (SPEI) in summer) over the years 1962–2011 (n = 50 years), the years with warm summers (early summer temperature above median (9.58 °C), n = 25) and the years with cool summers (early summer temperature below median, n = 25)
(n = 50 years)
(n = 25 years)
(n = 25 years)
Temperature variables (°C)
Length of growing season
Start date of growing season
Early summer temperature
Previous year’s summer temperature
Previous year’s early summer temperature
Precipitation variables (mm)
Previous year’s summer precipitation
Mean snow depth (cm)
Prev. SPEI June–August
SPEI previous year’s June–August
Precipitation also influenced radial growth patterns, but only in the years with a warm summer (Table 1). Annual radial growth rates were significantly correlated with both current-year and previous-year summer precipitation (Table 1) when the early summer temperature was above 9.58 °C (the median during the 50 years, 1962–2011). In the years with a below median early summer temperature, only early summer temperature was significantly related to RWI and no other climatic variable (Table 1). The mean snow depth did not significantly correlate with radial growth (Table 1). The relationship between SPEI and B. nana RWI values were significant only in the years with warm summers (Table 1, r were between 0.41 and 0.51, p < 0.05). SPEI over the period previous-year June up to current-year August had the largest correlation coefficient (r = 0.51, p = 0.010, n = 25 years), with high SPEI values indicating wet climatic conditions, taking evapotranspiration into account, corresponding with high ring widths.
Influence of climate factors on ramet establishment
Most of the 90 collected B. nana ramets established 20–50 years before sampling, while only five successfully established in the last 20 years, and the 10 oldest ramets initiated growth before 1963. The increase in establishment over the 1950s and 1960s coincided with increasing summer precipitation and SPEI over the same period (Fig. 4). Similarly, the dip in establishment numbers in the late 1970s was related to negative summer SPEI values indicating dry climatic conditions (Fig. 4). Establishment numbers were low since the mid-1990s in a period of declining precipitation. The 5-year establishment numbers over the period 1951–2000 were significantly related to June–August SPEI and precipitation, but were not related to temperature or temperature-related variables (SPEI June–August: p = 0.003, AIC = 58.4; summer precipitation: p = 0.018, AIC = 61.6; early summer temperature: p = 0.143, AIC = 64.8).
Climatic change and ramet establishment
In this study, a large number of B. nana samples were successfully cross-dated to build on a study by Blok et al. (2011a, b) on this widely spread and hence important Arctic shrub species. Although ring detection is sometimes difficult due to eccentric growth and partly missing rings, we succeeded in building a reliable B. nana site chronology with high rbar and EPS values.
The possibility of cross-dating the ring-width series of all ramets enabled reconstruction of the dynamics of ramet establishment across shrub patches. Although the sampling strategy was not specifically designed for analyzing ramet establishment in relation to climatic variations, we did obtain a dataset of 90 aged B. nana ramets that required measuring multiple radii in 3 stem sections per ramet and use cross-dating to obtain reliable ages in this difficult species with partial missing rings. The sampling was not directed to sample the thicker ramets as the primary purpose was to investigate age distribution patterns within the shrub patches. We sampled along transects from the center to the margin of the shrub patch. There was no relationship between age and position within the transects, and also the ages of the three center positions, which were relatively close to each other, were highly variable. This lack of an age pattern suggests that we probably sampled a random sample and not specific age cohorts. However, it was striking that the temporal distribution of the ramet establishment was not random. It is clear that most ramets established in periods with high summer precipitation around 1970 and in the 1980s, while the old and young (established before 1960 or after 1995) were rare, following the significant decline in summer precipitation since 1991 (slope = 4.56 mm year−1, R2 = 0.54, n = 20 years, n = 20 years: 1991–2010). This suggests that more ramets established when summer precipitation was high. Soil moisture supply in B. nana patches is controlled by summer precipitation and depth of permafrost thaw (Lloyd et al. 2003). As a shrub species with a highly plastic growing strategy (Bret-Harte et al. 2008), it is plausible that B. nana shrubs prefer to sprout new ramets when soil moisture is adequate, at the cost of stem growth (both radial and vertical). This may also explain the relatively low correlation between the radial growth curves and the year-to-year summer precipitation in our study. The continuously decreasing summer precipitation during the last two decades could be responsible for the low number of young ramets, i.e., recently established ramets among all samples.
We assume that the bias of missing old B. nana samples or young ramets during collection is low. Like many Arctic shrubs, B. nana shrub ramets can easily survive longer than 50 years (Groot et al. 1997). Dead stems are well preserved for years at our sites, due to the low decomposition rates of recalcitrant low-quality woody material in the harsh climate. Nevertheless, we found very few dead B. nana ramet relics inside the patches. Furthermore, for the statistical analysis we excluded the periods in which the oldest and youngest ramets established as we cannot be sure that we missed young or old ramets subjectively.
The negative precipitation trend and associated low establishment together with a lack of a positive trend in annual ring-width index could imply that there is no shrub expansion at our study site. Unfortunately, the lack of a long-term positive trend in ring-width index does not necessarily mean that climate warming did not affect shrub radial growth. The dendrochronological methods, e.g., detrending, remove gradual changes over time, as they could be the result of ontogenic, e.g., age-related, trends and optimize the inter-annual variation for investigation of growth-limiting factors. Perhaps there was a declining trend because of aging compensated by a positive trend because of climate change; it is impossible to distinguish between the two. Support for no shrub expansion comes from satellite NDVI records showing no greening trend for the study region over 2000–2010 (Blok et al. 2015). Local permafrost degradation resulting in thaw pond development and drowning of shrubs could have prevented net shrub expansion (Nauta et al. 2015). The negative precipitation trend is not unique to the study area, but also occurred in some other locations, particularly in the High Arctic (Urban et al. 2014). We used the KNMI Climate Change Atlas tool (http://climexp.knmi.nl/atlas) to generate a map of summer (JJA) precipitation trends over the years 1991–2015 for the Arctic land based on observations (CRU TS 3.22 dataset). The map (online resource 3) shows that the trends in summer precipitation are far from homogenous in the Arctic. In our study region, the Indigirka delta, the mean regression relative summer precipitation (1991–2015), is −50 % per century, and the similar patterns also exist in other Arctic regions, e.g., Lena Delta, Canadian High Arctic (online resource 3), while some other areas show strong positive trends. The large variation of trends in summer precipitation in the Arctic further suggests that shrub expansion might not happen everywhere in the Arctic tundra. However, future precipitation is expected to show an increasing trend as the retreat of sea ice results in strongly increased evaporation and precipitation (Bintanja and Selten 2014).
Climatic change and shrub growth
Several experimental studies in the Arctic (Hobbie and Chapin III 1998; Mack et al. 2004; Wahren et al. 2005; Walker et al. 2006) pointed out that the growth of B. nana was stimulated by increasing air temperature during the growing season. Most dendrochronological studies on the Arctic shrubs also indicated summer temperature as the most important factor for shrub growth through lengthening of the growing season (Hallinger et al. 2010). Myers-Smith et al. (2015a) stated that the summer temperature variable is the most influential factor that affects the variation of Arctic shrub growth. Our study confirmed that year-to-year variations in summer temperature strongly influenced the annual radial growth rates and that the mean temperature of early summer (June 16–July 20) was a more important factor for the radial growth of B. nana than that of the late growing season (Blok et al. 2011a).
Evidence from both the sample measurement and the field observation supported this deduction that early summer temperature is more important than late summer temperature for the width of a ring formed in a given year. In most ramets collected on July 20 in 2012, we observed that the final row(s) of flatted fibers were already formed, indicating that the formation of the 2012-ring was almost completed by July 20 (online resource 4). This suggests radial growth of B. nana already ceased. Furthermore, the leaf color of B. nana usually starts to turn from green to yellow and red at the beginning of August at our site (online resource 5), indicating the degradation of chlorophyll (Addicott and Lyon 1973) and lower photosynthesis activities. It further explains why the length of growing season (number of days with mean daily temperature over 5 °C per year) was not strongly correlated with the radial growth of B. nana. Although the mean temperature is usually above 5 °C until the beginning of September, from this research it seems that the radial growth of B. nana finishes approximately 3–4 weeks earlier, at both the stem base (disk α) and the upper part of the stem (disk β and disk γ) (Fig. 1).
Summer precipitation not only stimulated the sprouting of B. nana ramets, but facilitated the annual radial growth rates of B. nana shrubs as well, but only during warm summers. While low radial growth rates occur when early summer temperatures are low, relatively warm summers positively affect radial growth rates of B. nana, provided that summer precipitation, either in the actual summer and/or the previous summer, is above average. Remarkable was the peak growth rate in 2011, which coincided with a warm and wet summer, whereas the preceding summer of 2010 was even warmer but also very dry, resulting in a rather modest radial growth (online resource 2). At a nearby treeline research site, 2011 was also an exceptional year with high photosynthetic rate (Liang et al. 2014). It was recently stated that apart from summer temperature, soil moisture is another important factor that largely affects the growth of shrubs in the Arctic (Myers-Smith et al. 2015a). They further suggest that the importance of soil moisture to the growth of Arctic shrubs increased during warm summers. The results of our study support this statement, due to the positive correlation between soil moisture and summer precipitation in the study region (Liang et al. 2014).
The effect of previous year’s summer precipitation on shrub growth is probably related to local soil nutrient supply. In the Arctic tundra, rapid growth of deciduous shrubs, for instance B. nana and S. pulchra, in the early growing season (late June to early July) is mainly supported by belowground nutrient storage instead of nutrient absorption at the time that the soil is still mostly frozen (Chapin III 1980; Chapin III et al. 1990), which is a common strategy among Arctic plants. Due to the extremely harsh winter environment (frozen soil and low temperature) in northeast Siberian tundra, the belowground nutrient accumulation mainly takes place during the previous growing season (Bilbrough et al. 2000). The soil nutrient absorption of the previous years is critical to the shrub growth of the next seasons. Higher soil moisture in the Arctic tundra might accelerate the permafrost thawing at B. nana-dominated patches during the growing season by increasing soil thermal conductivity. Six years of thaw depth measurements at the research site revealed that summer thaw depth was largest in 2011, which had exceptionally large summer precipitation (Nauta et al. 2015). The larger thaw depth might lead to a higher nutrient availability in deeper soil (Wahren et al. 2005). Dry soil conditions at the surface could reduce decomposition of organic matter, potentially reducing nutrient supply (Blok et al. 2015). Further studies should include investigations on soil nutrient availability. Since growth of deciduous shrubs like B. nana is highly sensitive to changes in soil nutrient availability (Marschner et al. 2007), inter-annual variations in B. nana growth may follow variations in soil nutrient availability (Shaver et al. 2001).
It has been widely shown that growth of deciduous shrubs like B. nana is responsive to changes in summer air temperature. This study builds on this result and further suggests that summer precipitation also influences growth rates, perhaps indirectly through soil nutrient availability; moreover, summer precipitation seems to play an important role for shrub establishment. This implies that summer precipitation can facilitate the expansion of B. nana in Arctic tundra and that the influence of precipitation dynamics on vegetation composition changes in high-latitude ecosystems should not be ignored or underestimated.
This study is financed by the Darwin Center for Biogeosciences, Wageningen Institute for Environment and Climate Research (WIMEK) and the Netherlands Organization for Scientific Research (NWO, Vidi Grant 864.09.014). Daan Blok was funded through a grant by the Danish National Research Foundation (CENPERM DNRF100). We kindly thank Stas Ksenofontov and other staff of the IBPC institute, Yakutsk, and staff of the Regional Inspection of Nature Protection of Allaikhovsky Region, Chokurdakh, for logistic support. We thank Jelmer Nijp, Natali Oram, Joost Huttenga and Marjolein Mann for their helpful comments on the manuscript. This study was inspired by discussion within the framework of the COST Action FP1106, STReESS.
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