Polar Biology

, Volume 29, Issue 8, pp 662–667

Dense populations of Archaea associated with the demosponge Tentorium semisuberites Schmidt, 1870 from Arctic deep-waters

  • Thomas Pape
  • Friederike Hoffmann
  • Nadia-Valérie Quéric
  • Karen von Juterzenka
  • Joachim Reitner
  • Walter Michaelis
Original Paper

DOI: 10.1007/s00300-005-0103-4

Cite this article as:
Pape, T., Hoffmann, F., Quéric, NV. et al. Polar Biol (2006) 29: 662. doi:10.1007/s00300-005-0103-4

Abstract

The associated microbial community in the mesohyl of the Arctic deep-water sponge Tentorium semisuberites Schmidt, 1870 (Hadromerida, Demospongiae) is dominated by Archaea. This is the result of an integral approach applying analyses of microbial lipid biomarkers as well as microscopic investigations using differential fluorescence in situ hybridisation with universal probes and counterstaining with 4′,6′-diamidino-2-phenylindole (DAPI) on sponge sections based on samples collected in the Greenland Sea in 2001, 2002 and 2005. The distribution of isoprenoidal C40 hydrocarbons of the biphytane series suggests that affiliates of both major archaeal kingdoms, the Crenarchaeota and the Euryarchaeota, are present in the choanosome of T. semisuberites. Positive signals using the oligonucleotide probe ARCH915 indicate high numbers of Archaea in the mesohyl of this sponge. Based on optical estimations 70–90% of all microbial DAPI signals accounted for archaeal cells. Archaea in these high proportions have never been described in an Arctic deep-sea hadromerid sponge, nor in any other demosponge species. Similar observations in specimens collected over a time scale of 4 years suggest permanent sponge-Archaea associations.

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Thomas Pape
    • 1
    • 6
  • Friederike Hoffmann
    • 2
    • 3
  • Nadia-Valérie Quéric
    • 4
  • Karen von Juterzenka
    • 4
    • 5
  • Joachim Reitner
    • 2
  • Walter Michaelis
    • 1
  1. 1.Institute of Biogeochemistry and Marine ChemistryUniversity of HamburgHamburgGermany
  2. 2.Center of Geosciences, GeobiologyUniversity of GöttingenGöttingenGermany
  3. 3.Max Planck Institute for Marine MicrobiologyBremenGermany
  4. 4.Alfred-Wegener-Institute for Polar and Marine Research, Deep Sea ResearchBremerhavenGermany
  5. 5.Institute for Polar EcologyChristian-Albrechts-University of KielKielGermany
  6. 6.Research Center Ocean MarginsUniversity of BremenBremenGermany