Plant Cell Reports

, Volume 18, Issue 3, pp 187–192

In vitro propagation, restriction fragment length polymorphism, and random amplified polymorphic DNA analyses of Angelica plants

  • A. Watanabe
  • S. Araki
  • S. Kobari
  • H. Sudo
  • T. Tsuchida
  • T. Uno
  • N. Kosaka
  • K. Shimomura
  • M. Yamazaki
  • K. Saito

DOI: 10.1007/s002990050554

Cite this article as:
Watanabe, A., Araki, S., Kobari, S. et al. Plant Cell Reports (1998) 18: 187. doi:10.1007/s002990050554

Abstract

Angelica acutiloba, a medicinal plant used as a natural medicine Touki, was clonally propagated through axillary buds in vitro. No substantial differences were found in the random amplified polymorphic DNA (RAPD) pattern between the original A. acutiloba and the plant propagated in vitro, suggesting no changes in the DNA sequences and structure during in vitro propagation. The genetic similarities of several Angelica plants were investigated by restriction fragment length polymorphism (RFLP) and RAPD analyses. The RFLP and RAPD patterns of A. sinensis Diels were substantially different from those of A. acutiloba. Using ten different restriction enzymes, no RFLP was observed in the varieties of A. acutiloba. By RAPD analysis, A. acutiloba varieties can be classified into two major subgroups, i.e., A. acutiloba Kitagawa and A. acutiloba Kitagawa var. sugiyamae Hikino. The varieties of A. acutiloba Kitagawa in Japan and Angelica spp. in northeast China exhibited a very close genetic relationship.

Key wordsAngelicaRestriction fragment length polymorphismRandom amplified polymorphic DNALigustilideIn vitro propagation

Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • A. Watanabe
    • 1
  • S. Araki
    • 1
  • S. Kobari
    • 1
  • H. Sudo
    • 2
  • T. Tsuchida
    • 3
  • T. Uno
    • 3
  • N. Kosaka
    • 3
  • K. Shimomura
    • 4
  • M. Yamazaki
    • 1
  • K. Saito
    • 1
  1. 1.Faculty of Pharmaceutical Sciences, Research Center of Medicinal Resources, Laboratory of Molecular Biology and Biotechnology, Chiba University, Yayoi-cho 1-33, Inage-ku, Chiba 263-8522, Japan e-mail: ksaito@p.chiba-u.ac.jp Fax: +81-43-2902905JP
  2. 2.Basic Research Laboratory, Kanebo Co. Ltd., Tomobuchi-cho 1-5-90, Miyakojima-ku, Osaka 534-0016, JapanJP
  3. 3.Kampo Research Laboratory, Kanebo Co. Ltd., Tomobuchi-cho 1-5-90, Miyakojima-ku, Osaka 534-0016, JapanJP
  4. 4.Tsukuba Medicinal Plant Research Station, National Institute of Health Science, Hachimandai 1, Tsukuba, Ibaraki 305-0843, JapanJP