Plant Cell Reports

, Volume 17, Issue 12, pp 946–950

Regulation of polyphenol production in Vitis vinifera cell suspension cultures by sugars

  • F. Larronde
  • S. Krisa
  • A. Decendit
  • C. Chèze
  • G. Deffieux
  • J. M. Mérillon

DOI: 10.1007/s002990050515

Cite this article as:
Larronde, F., Krisa, S., Decendit, A. et al. Plant Cell Reports (1998) 17: 946. doi:10.1007/s002990050515

Abstract

Sucrose was found to modulate polyphenol accumulation in Vitis vinifera cell cultures. The production of anthocyanins increased 12-fold after addition of 0.15 m sucrose, while that of stilbenes was only slightly affected. Sucrose did not play a physical role because metabolic sugars were required for the induction of polyphenol accumulation. Indeed, the polyols, mannitol and sorbitol, had no effect on this accumulation. We established a model system to investigate the mechanism of sucrose regulation of polyphenol production without inhibition of grape cell growth. After addition of sucrose to the culture medium, the major sugars accumulated in grape cells were glucose and fructose, reaching 40% of the dry weight. The increase in the level of these hexoses closely coincided with the increase in anthocyanin accumulation in grape cells.

Key wordsVitis viniferaCell suspension cultureStilbenesAnthocyaninsSugars

Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • F. Larronde
    • 1
  • S. Krisa
    • 1
  • A. Decendit
    • 1
  • C. Chèze
    • 1
  • G. Deffieux
    • 1
  • J. M. Mérillon
    • 1
  1. 1.Groupe d'Etude des Substances Naturelles à Intérêt Thérapeutique, EA 491, Faculté de Pharmacie, Université de Bordeaux II, 3 place de la Victoire, F-33000 Bordeaux, France Fax: +33-5-56917988 e-mail: jean-michel.merillon@phyto.u-bordeaux2.frFR