Plant Cell Reports

, Volume 17, Issue 5, pp 345–355

Rapid and efficient transformation of diploid Medicago truncatula and Medicago sativa ssp. falcata lines improved in somatic embryogenesis

  • T. H. Trinh
  • P. Ratet
  • E. Kondorosi
  • P. Durand
  • K. Kamaté
  • P. Bauer
  • A. Kondorosi

DOI: 10.1007/s002990050405

Cite this article as:
Trinh, T., Ratet, P., Kondorosi, E. et al. Plant Cell Reports (1998) 17: 345. doi:10.1007/s002990050405

Abstract

We describe a simple and efficient protocol for regeneration-transformation of two diploid Medicago lines: the annual M. truncatula R108-1(c3) and the perennial M. sativa ssp. falcata (L.) Arcangeli PI.564263 selected previously as highly embryogenic genotypes. Here, embryo regeneration of R108-1 to complete plants was further improved by three successive in vitro regeneration cycles resulting in the line R108-1(c3). Agrobacterium tumefaciens-mediated transformation of leaf explants was carried out with promoter-gus constructs of two early nodulins (MsEnod12A and MsEnod12B) and one late nodulin (Srglb3). The transgenic plants thus produced on all explants within 3–4 months remained diploid and were fertile. This protocol appears to be the most efficient and fastest reported so far for leguminous plants.

Key wordsM. truncatulaM. falcataMsEnod12AMsEnod12BSrglb3

Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • T. H. Trinh
    • 1
  • P. Ratet
    • 1
  • E. Kondorosi
    • 1
  • P. Durand
    • 1
  • K. Kamaté
    • 1
  • P. Bauer
    • 1
  • A. Kondorosi
    • 1
  1. 1.Institut des Sciences Végétales, Centre National de la Recherche Scientifique, UPR40, Avenue de la terrasse, F-91198 Gif-sur-Yvette Cedex, France Fax no.: +33-1-69823695FR