Plant Cell Reports

, Volume 16, Issue 12, pp 831–835

Micropropagation of Spathoglottis plicata

  • W.-L. Teng
  • L. Nicholson
  • M.-C. Teng

DOI: 10.1007/s002990050329

Cite this article as:
Teng, WL., Nicholson, L. & Teng, MC. Plant Cell Reports (1997) 16: 831. doi:10.1007/s002990050329

Abstract

A rapid and reliable micropropagation method was established for Spathoglottis plicata. Nodal and leaf explants dissected from 8-month-old pot-grown seedlings were cultured on charcoal-amended Murashige and Skoog medium supplemented with 16 combinations of α-naphthaleneacetic acid (NAA) and 6-benzylaminopurine (BA) at concentrations of 0.54–10.74 µm. Regeneration of protocorm-like bodies (PLBs) and subsequent plantlet development were observed from 98.5% of the nodal explants. Only 6.5% of leaf explants and occasionally some root segments (dissected from regenerated plantlets) were able to produce PLBs and then plantlets. The optimum plant growth regulator (PGR) combination for maximal PLB regeneration was 5.37 µm NAA and 0.44 µm BA. The best combination of PGR for plantlet development was 2.69–10.74 µm NAA and 8.88 µm BA. The NAA to BA ratios for maximal PLB induction and plantlet development were 12.2 and 0.3–1.2, respectively. Regenerated PLBs and plantlets, when cut into pieces of less than 1 mm and subcultured onto the above media, regenerated new PLBs and plantlets in another 3 months.

Key words Organogenesis Micropropagation Protocorm-like-body Orchid 

Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • W.-L. Teng
    • 1
  • L. Nicholson
    • 1
  • M.-C. Teng
    • 2
  1. 1.Hong Kong Institute of Biotechnology, 2 Biotechnology Avenue, 12 Miles, Tai Po Road, Shatin, N. T., Hong Kong E-mail: wl-teng@hkib.org.hkHK
  2. 2.Department of Plant Industry, National Pingtung Polytechnic Institute, 1 Hseuh Fu Road., Nei Pu Hsiang, Pingtung, Taiwan; Fax no.: +852-2603-6820TW

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