Plant Cell Reports

, Volume 19, Issue 3, pp 315–320

An Agrobacterium tumefaciens transformation protocol effective on a variety of cottonwood hybrids (genus Populus)

  • K.-H. Han
  • R. Meilan
  • C. Ma
  • S. H. Strauss
Original paper

DOI: 10.1007/s002990050019

Cite this article as:
Han, KH., Meilan, R., Ma, C. et al. Plant Cell Reports (2000) 19: 315. doi:10.1007/s002990050019

Abstract

 We describe a protocol for Agrobacterium tumefaciens-mediated transformation of hybrid cottonwoods (Populus sections Tacamahaca Spach. and Aigeiros Duby). The protocol has allowed routine transformation of several economically important cottonwood hybrids (Populus trichocarpa Torr. & Gray×P. deltoides Bartr. ex. Marsh. and P. deltoides×P. nigra L.) that were previously difficult to transform. The procedure was applied to 11 different hybrid cottonwood genotypes and one P. deltoides genotype using kanamycin as the selection agent. Additional experiments showed a very strong interaction between auxin preculture and the effectiveness of various cytokinins for induction of shoot organogenesis. The data also demonstrated the superiority of Agrobacterium strain EHA105 over C58 and LBA4404 for T-DNA transfer based on transient assays with a reporter gene.

Key words PoplarPopulusAspenCottonwoodTransformationAgrobacterium

Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • K.-H. Han
    • 1
  • R. Meilan
    • 1
  • C. Ma
    • 1
  • S. H. Strauss
    • 1
  1. 1.Department of Forest Science, Oregon State University, Corvallis, OR 97331–7501, USA e-mail: strauss@FSL.orst.edu Fax: +541–737–1393US