GENETIC TRANSFORMATION AND HYBRIDIZATION

Plant Cell Reports

, Volume 20, Issue 3, pp 220-226

First online:

Intron-mediated enhancement of the banana bunchy top virus DNA-6 promoter in banana (Musa spp.) embryogenic cells and plants

  • B. DugdaleAffiliated withCentre for Molecular Biotechnology, School of Life Sciences, Queensland University of Technology, GPO Box 2434, Brisbane, Queensland, Australia 4001 e-mail: j.dale@qut.edu.au Fax: +61-7-38641534
  • , D. K. BeckerAffiliated withCentre for Molecular Biotechnology, School of Life Sciences, Queensland University of Technology, GPO Box 2434, Brisbane, Queensland, Australia 4001 e-mail: j.dale@qut.edu.au Fax: +61-7-38641534
  • , R. M. HardingAffiliated withCentre for Molecular Biotechnology, School of Life Sciences, Queensland University of Technology, GPO Box 2434, Brisbane, Queensland, Australia 4001 e-mail: j.dale@qut.edu.au Fax: +61-7-38641534
  • , J. L. DaleAffiliated withCentre for Molecular Biotechnology, School of Life Sciences, Queensland University of Technology, GPO Box 2434, Brisbane, Queensland, Australia 4001 e-mail: j.dale@qut.edu.au Fax: +61-7-38641534

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Abstract

 Intron-containing fragments derived from the 5′ untranslated regions of the maize ubi1, maize adh1, rice act1 and sugarcane rbcS genes were tested for their enhancing effects on the banana bunchy top virus DNA-6 promoter (BT6.1) in banana (Musa spp. cv. Bluggoe) embryogenic cells. The rice act1 and maize ubi1 introns provided the highest levels of intron-mediated enhancement of GUS expression, increasing native BT6.1 promoter activity by about 300-fold and 100-fold, respectively. The sugarcane rbcS intron increased expression about tenfold, whereas the adh1 intron had no significant effect. In regenerated transgenic banana plants, the ubi1 intron significantly enhanced BT6.1 promoter activity to levels similar to that of the CaMV 35 S promoter and did not appear to affect the tissue specificity of the promoter.

Keywords Banana bunchy top virus Promoter Intron-mediated enhancement GUS