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Identification of quantitative trait loci and candidate genes for specific cellular resistance responses against Didymella pinodes in pea

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Phenotyping of specific cellular resistance responses and improvement of previous genetic map allowed the identification of novel genomic regions controlling cellular mechanisms involved in pea resistance to ascochyta blight and provided candidate genes suitable for MAS.

Abstract

Didymella pinodes, causing ascochyta blight, is a major pathogen of the pea crop and is responsible for serious damage and yield losses. Resistance is inherited polygenically and several quantitative trait loci (QTLs) have been already identified. However, the position of these QTLs should be further refined to identify molecular markers more closely linked to the resistance genes. In previous works, resistance was scored visually estimating the final disease symptoms; in this study, we have conducted a more precise phenotyping of resistance evaluating specific cellular resistance responses at the histological level to perform a more accurate QTL analysis. In addition, P665 × Messire genetic map used to identify the QTLs was improved by adding 117 SNP markers located in genes. This combined approach has allowed the identification, for the first time, of genomic regions controlling cellular mechanisms directly involved in pea resistance to ascochyta blight. Furthermore, the inclusion of the gene-based SNP markers has allowed the identification of candidate genes co-located with QTLs and has provided robust markers for marker-assisted selection.

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Acknowledgments

E. Carrillo was granted by a Cabildo de La Palma-CSIC PhD grant and S. Fondevilla by a MC contract: FP7-PEOPLE-2011-IEF-300235. Financial support by projects AGL2011-22524 (co-financed by FEDER) and FP7-ARIMNet-Medileg is acknowledged. The authors wish to thank Sophie Valière and Cécile Donnadieu for the use of the GeT-PlaGe Genotyping platform and their expertise.

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical standards

The experiments comply with the current laws of the country in which they were performed.

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Authors and Affiliations

  1. Institute for Sustainable Agriculture, CSIC, Apdo. 4084, 14080, Córdoba, Spain

    E. Carrillo & D. Rubiales

  2. Department of Seed Science and Technology, Faculty of Agriculture, University of Zagreb, Svetošimunska 25, 10000, Zagreb, Croatia

    Z. Satovic

  3. INRA UMRLEG, BP 86510, 21065, Dijon, France

    G. Aubert & K. Boucherot

  4. Institut für Moleculare Biowissenschaften, Johann Wolfgang Goethe University, Frankfurt, Germany

    S. Fondevilla

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  1. E. Carrillo
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  2. Z. Satovic
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  3. G. Aubert
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  4. K. Boucherot
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  5. D. Rubiales
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  6. S. Fondevilla
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Correspondence to E. Carrillo.

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Communicated by P. Puigdomenech.

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Carrillo, E., Satovic, Z., Aubert, G. et al. Identification of quantitative trait loci and candidate genes for specific cellular resistance responses against Didymella pinodes in pea. Plant Cell Rep 33, 1133–1145 (2014). https://doi.org/10.1007/s00299-014-1603-x

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