Plant Cell Reports

, Volume 32, Issue 1, pp 129–137

The interaction of banana MADS-box protein MuMADS1 and ubiquitin-activating enzyme E-MuUBA in post-harvest banana fruit

  • Ju-Hua Liu
  • Jing Zhang
  • Cai-Hong Jia
  • Jian-Bin Zhang
  • Jia-Shui Wang
  • Zi-Xian Yang
  • Bi-Yu Xu
  • Zhi-Qiang Jin
Original Paper

DOI: 10.1007/s00299-012-1347-4

Cite this article as:
Liu, JH., Zhang, J., Jia, CH. et al. Plant Cell Rep (2013) 32: 129. doi:10.1007/s00299-012-1347-4

Abstract

Key message

The interaction of MuMADS1 and MuUBA in banana was reported, which will help us to understand the mechanism of the MADS-box gene in regulating banana fruit development and ripening.

Abstract

The ubiquitin-activating enzyme E1 gene fragment MuUBA was obtained from banana (Musa acuminata L.AAA) fruit by the yeast two-hybrid method using the banana MADS-box gene MuMADS1 as bait and 2-day post-harvest banana fruit cDNA library as prey. MuMADS1 interacted with MuUBA. The interaction of MuMADS1 and MuUBA in vivo was further proved by bimolecular fluorescence complementation assay. Real-time quantitative PCR evaluation of MuMADS1 and MuUBA expression patterns in banana showed that they are highly expressed in the ovule 4 stage, but present in low levels in the stem, which suggests a simultaneously differential expression action exists for both MuMADS1 and MuUBA in different tissues and developmental fruits. MuMADS1 and MuUBA expression was highly stimulated by exogenous ethylene and suppressed by 1-methylcyclopropene. These results indicated that MuMADS1 and MuUBA were co-regulated by ethylene and might play an important role in post-harvest banana fruit ripening.

Keywords

Banana (Musa acuminata L. AAA group, cv. Brazilian)Yeast two-hybridMuMADS1MuUBAInteractionPost-harvest ripening

Copyright information

© Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • Ju-Hua Liu
    • 1
  • Jing Zhang
    • 1
  • Cai-Hong Jia
    • 1
  • Jian-Bin Zhang
    • 1
  • Jia-Shui Wang
    • 2
  • Zi-Xian Yang
    • 1
  • Bi-Yu Xu
    • 1
  • Zhi-Qiang Jin
    • 1
    • 2
  1. 1.Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture; Institute of Tropical Bioscience and BiotechnologyChinese Academy of Tropical Agricultural SciencesHaikouChina
  2. 2.Haikou Experimental StationChinese Academy of Tropical Agricultural SciencesHaikouChina