Plant Cell Reports

, Volume 31, Issue 1, pp 67–79

Molecular characterization of a rice metal tolerance protein, OsMTP1

  • Lianyu Yuan
  • Songguang Yang
  • Baoxiu Liu
  • Mei Zhang
  • Keqiang Wu
Original Paper

DOI: 10.1007/s00299-011-1140-9

Cite this article as:
Yuan, L., Yang, S., Liu, B. et al. Plant Cell Rep (2012) 31: 67. doi:10.1007/s00299-011-1140-9

Abstract

Rice (Oryza sativa L. ‘Nipponbare’) cDNA subtractive suppression hybridization (SSH) libraries constructed using cadmium (Cd)-treated seedling roots were screened to isolate Cd-responsive genes. A cDNA clone, encoding the rice homolog of Metal Tolerance Protein (OsMTP1), was induced by Cd treatment. Plant MTPs belong to cation diffusion facilitator (CDF) protein family, which are widespread in bacteria, fungi, plants, and animals. OsMTP1 heterologous expression in yeast mutants showed that OsMTP1 was able to complement the mutant strains’ hypersensitivity to Ni, Cd, and Zn, but not other metals including Co and Mn. OsMTP1 expression increased tolerance to Zn, Cd, and Ni in wild-type yeast BY4741 during the exponential growth phase. OsMTP1 fused to green fluorescent protein was localized in onion epidermal cell plasma membranes, consistent with an OsMTP1 function in heavy metal transporting. OsMTP1 dsRNAi mediated by transgenic assay in rice seedlings resulted in heavy metal sensitivity and changed the heavy metal accumulation in different organs of mature rice under low-concentration heavy metal stress. Taken together, our results show that OsMTP1 is a bivalent cation transporter localized in the cell membrane, which is necessary for efficient translocation of Zn, Cd and other heavy metals, and maintain ion homeostasis in plant.

Keywords

Metal tolerance proteinHeavy metalRice

Abbreviations

MTP

Metal tolerance protein

CDF

Cation diffusion facilitator

NRAMP

Natural resistance-associated macrophage protein

ZIP

Zn-regulated transporter-/Fe-regulated transporter-like protein

TMD

Transmembrane domain

ZAT

Zinc transporter of Arabidopsis thaliana

AAS

Atomic absorption spectrometer

dsRNAi

Double-stranded RNA interference

SD medium

Synthetic defined medium

RT-PCR

Reverse transcription PCR

DIG

Digoxigenin

ORF

Open reading frame

EGFP

Enhanced green fluorescent protein

SSH

Subtractive suppression hybridization

ISH

In situ hybridization

Supplementary material

299_2011_1140_MOESM1_ESM.doc (60 kb)
Supplement data 1 (DOC 60 kb)
299_2011_1140_MOESM2_ESM.doc (27 kb)
Supplement data 2 (DOC 27 kb)
299_2011_1140_MOESM3_ESM.doc (34 kb)
Supplement data 3 (DOC 34 kb)

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Lianyu Yuan
    • 1
    • 2
  • Songguang Yang
    • 1
  • Baoxiu Liu
    • 1
    • 2
  • Mei Zhang
    • 1
  • Keqiang Wu
    • 1
  1. 1.Key Laboratory of Plant Resources Conservation and Sustainable UtilizationSouth China Botanical Garden, Chinese Academy of SciencesGuangzhouPeople’s Republic of China
  2. 2.Graduate School of the Chinese Academy of SciencesBeijingPeople’s Republic of China