Plant Cell Reports

, Volume 30, Issue 12, pp 2243–2254

Heading date gene, dth3 controlled late flowering in O. Glaberrima Steud. by down-regulating Ehd1

  • X. F. Bian
  • X. Liu
  • Z. G. Zhao
  • L. Jiang
  • H. Gao
  • Y. H. Zhang
  • M. Zheng
  • L. M. Chen
  • S. J. Liu
  • H. Q. Zhai
  • J. M. Wan
Original Paper

DOI: 10.1007/s00299-011-1129-4

Cite this article as:
Bian, X.F., Liu, X., Zhao, Z.G. et al. Plant Cell Rep (2011) 30: 2243. doi:10.1007/s00299-011-1129-4

Abstract

Heading date in rice is an important agronomic trait controlled by several genes. In this study, flowering time of variety Dianjingyou 1 (DJY1) was earlier than a near-isogenic line (named NIL) carried chromosome segment from African rice on chromosome 3S, when grown in both long-day (LD) and short-day (SD) conditions. By analyzing a large F2 population from NIL × DJY1, the locus DTH3 (QTL for days to heading on chromosome 3) controlling early heading date in DJY1 was fine mapped to a 64-kb segment which contained only one annotated gene, a MIKC-type MADS-box protein. We detected a 6-bp deletion and a single base substitution in the C-domain by sequencing DTH3 in DJY1 compared with dth3 in NIL, and overexpression of DTH3 caused early flowering in callus. Quantitative real-time PCR revealed that the transcript level of dth3 in NIL was lower than that DTH3 in DJY1 in both LD and SD conditions. The Early heading date 1 (Ehd1) which promotes the RFT1, was up-regulated by DTH3 in both LD and SD conditions. Based on Indel and dCAPs marker analysis, the dth3 allele was only present in African rice accessions. A phylogenetic analysis based on microsatellite genotyping suggested that African rice had a close genetic relationship to O. rufipogon and O. latifolia, and was similar to japonica cultivars. DTH3 affected flowering time and had no significant effect on the main agronomic traits.

Keywords

Near-isogenic linesDays to headingFlowering regulatorAfrican rice MADS-box

Abbreviations

DJY1

Dianjingyou 1

Ehd1

Early heading date 1

LD

Long-day

MAS

Marker-assisted selection

NILs

Near-isogenic lines

SD

Short-day

SSR

Simple sequence repeat

Supplementary material

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Supplementary Fig. 1 (DOC 100 kb)
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Supplementary Fig. 2 (DOC 87 kb)
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Supplementary Fig. 3 (DOC 30 kb)
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Supplementary Fig. 5 (DOC 29 kb)
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Supplementary Table 1 (DOC 46 kb)
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Supplementary Table 2 (DOC 50 kb)
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Supplementary Table 3 (DOC 526 kb)

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • X. F. Bian
    • 1
  • X. Liu
    • 1
  • Z. G. Zhao
    • 1
  • L. Jiang
    • 1
  • H. Gao
    • 1
  • Y. H. Zhang
    • 1
  • M. Zheng
    • 1
  • L. M. Chen
    • 1
  • S. J. Liu
    • 1
  • H. Q. Zhai
    • 2
  • J. M. Wan
    • 1
    • 2
  1. 1.National Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research CenterNanjing Agricultural UniversityNanjingChina
  2. 2.Institute of Crop Science, The National Key Facility for Crop Gene Resources and Genetic ImprovementChinese Academy of Agricultural Sciences (CAAS)BeijingChina