Plant Cell Reports

, Volume 30, Issue 1, pp 101–112

Expression of a barley cystatin gene in maize enhances resistance against phytophagous mites by altering their cysteine-proteases

  • Laura Carrillo
  • Manuel Martinez
  • Koreen Ramessar
  • Inés Cambra
  • Pedro Castañera
  • Felix Ortego
  • Isabel Díaz
Original Paper

DOI: 10.1007/s00299-010-0948-z

Cite this article as:
Carrillo, L., Martinez, M., Ramessar, K. et al. Plant Cell Rep (2011) 30: 101. doi:10.1007/s00299-010-0948-z

Abstract

Phytocystatins are inhibitors of cysteine-proteases from plants putatively involved in plant defence based on their capability of inhibit heterologous enzymes. We have previously characterised the whole cystatin gene family members from barley (HvCPI-1 to HvCPI-13). The aim of this study was to assess the effects of barley cystatins on two phytophagous spider mites, Tetranychus urticae and Brevipalpus chilensis. The determination of proteolytic activity profile in both mite species showed the presence of the cysteine-proteases, putative targets of cystatins, among other enzymatic activities. All barley cystatins, except HvCPI-1 and HvCPI-7, inhibited in vitro mite cathepsin L- and/or cathepsin B-like activities, HvCPI-6 being the strongest inhibitor for both mite species. Transgenic maize plants expressing HvCPI-6 protein were generated and the functional integrity of the cystatin transgene was confirmed by in vitro inhibitory effect observed against T. urticae and B. chilensis protein extracts. Feeding experiments impaired on transgenic lines performed with T. urticae impaired mite development and reproductive performance. Besides, a significant reduction of cathepsin L-like and/or cathepsin B-like activities was observed when the spider mite fed on maize plants expressing HvCPI-6 cystatin. These findings reveal the potential of barley cystatins as acaricide proteins to protect plants against two important mite pests.

Keywords

Cysteine protease Phytocystatin Spider mite Transgenic maize Tetranychus urticae Brevipalpus chilensis 

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Laura Carrillo
    • 1
    • 3
  • Manuel Martinez
    • 3
  • Koreen Ramessar
    • 2
  • Inés Cambra
    • 3
  • Pedro Castañera
    • 1
  • Felix Ortego
    • 1
  • Isabel Díaz
    • 3
  1. 1.Dpto. Biología MedioambientalCentro de Investigaciones Biológicas, CSICMadridSpain
  2. 2.Departament de Producció Vegetal i Ciència ForestalETSEA, Universitat de LleidaLleidaSpain
  3. 3.Centro de Biotecnología y Genómica de Plantas, Campus Montegancedo Universidad Politécnica de MadridMadridSpain

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