Plant Cell Reports

, Volume 29, Issue 2, pp 153–161

Mycotoxin Ochratoxin A-induced cell death and changes in oxidative metabolism of Arabidopsis thaliana

Original Paper

DOI: 10.1007/s00299-009-0808-x

Cite this article as:
Peng, X., Xu, W., Wang, Y. et al. Plant Cell Rep (2010) 29: 153. doi:10.1007/s00299-009-0808-x

Abstract

We evaluated the phytotoxicity of mycotoxin ochratoxin A (OTA) from Aspergillus and Penicillium strains on Arabidopsis thaliana. The results demonstrate that the growth of Arabidopsis thaliana on media containing OTA was inhibited significantly. Moreover, OTA induced necrotic lesions in detached leaves, which are reminiscent of hypersensitive response lesions that are activated during plant–pathogen interactions and other abiotic stress factors. From our study, we can see that OTA exposure stimulated a biphasic oxidative burst in the leaves, resulting in the generation of hydrogen peroxide (H2O2) and superoxide anion radicals (O2·−) and in the concomitant down-regulation of antioxidant enzyme defense responses and up-regulation of lipid peroxidation. These results suggested that OTA damage might result from reactive oxygen species pathways. Our experiments provide a useful model plant system for research on OTA-induced plant cell death.

Keywords

Arabidopsis thalianaOchratoxin ACell deathReactive oxygen species

Abbreviations

APX

Ascorbate peroxidases

CAT

Catalases

DAB

3,3′-Diaminobenzidine

DON

Deoxynivalenol

FB1

Fumonisin B1

H2DCFDA

2′,7′-Dichlorodihydrofluorescein diacetate

H2O2

Hydrogen peroxide

HR

Hypersensitive response

MDA

Malondialdehyde

MES

2-(N-morpholino) ethanesulphonic acid

MS

Murashige and Skoog

NBT

Nitroblue tetrazolium

O2·−

Superoxide anion radicals

OTA

Ochratoxin A

PCD

Programmed cell death

POD

Peroxidase

PR

Pathogenesis related

ROS

Reactive oxygen species

SOD

Superoxide dismutase

Supplementary material

299_2009_808_MOESM1_ESM.doc (150 kb)
Supplementary material 1 (DOC 149 kb)

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  1. 1.Laboratory of Fruit Physiology and Molecular Biology, College of Food Science and Nutritional EngineeringChina Agricultural UniversityBeijingChina
  2. 2.Supervision and Testing Center of Agricultural Products QualityMinistry of AgricultureBeijingChina