Plant Cell Reports

, Volume 28, Issue 6, pp 891–902

MicroRNA expression profiles in conventional and micropropagated strawberry (Fragaria × ananassa Duch.) plants

  • He Li
  • Zhihong Zhang
  • Feifei Huang
  • Linlin Chang
  • Yue Ma
Cell Biology and Morphogenesis

DOI: 10.1007/s00299-009-0693-3

Cite this article as:
Li, H., Zhang, Z., Huang, F. et al. Plant Cell Rep (2009) 28: 891. doi:10.1007/s00299-009-0693-3

Abstract

MicroRNAs (miRNAs) are a class of small non-coding RNAs which play a critical role in plant growth and development. To detect strawberry miRNAs and discover the expression difference between conventional and micropropagated strawberry plants, we carried out the detection and quantification of strawberry miRNAs by microarray. The main findings were that 74 miRNAs were checked in strawberry plants and four miRNA genes displayed clear expression difference between conventional and micropropagated strawberry plants, including two up-regulated genes (miR535 and miR390) and two down-regulated genes (miR169a and miR169d). The ratios of conventionally propagated strawberry plant/micropropagated strawberry plant for miR535, miR390, miR169a and miR169d were 2.6884, 2.2673, 0.2496 and 0.3814, respectively. Quantitative reverse transcription polymerase chain reaction applied to the two up-regulated genes (miR535 and miR390) validated the microarray result. This is the first report on differential expression of miRNAs in conventional and micropropagated plants.

Keywords

MicroRNA Microarray Q-RT-PCR Fragaria × ananassa Micropropagation 

Abbreviations

IBA

Indole-3-butyric acid

RT

Reverse transcription

PCR

Polymerase chain reaction

RT-PCR

Reverse transcription-polymerase chain reaction

Q-RT-PCR

Quantitative-reverse transcription-polymerase chain reaction

cDNA

Complementary deoxyribonucleic acid

Supplementary material

299_2009_693_MOESM1_ESM.xls (128 kb)
Supplementary material 1 (XLS 128 kb)

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • He Li
    • 1
  • Zhihong Zhang
    • 1
  • Feifei Huang
    • 1
  • Linlin Chang
    • 1
  • Yue Ma
    • 1
  1. 1.College of HorticultureShenyang Agricultural UniversityShenyangPeople’s Republic of China

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